Chemical Composition and Cytotoxicity of Kalanchoe pinnata Leaves Extracts prepared using Accelerated System Extraction (ASE)

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dc.citation.issue2
dc.citation.volume13
dc.contributor.authorPereira, Kassia M. F. [UNIFESP]
dc.contributor.authorGrecco, Simone S.
dc.contributor.authorFigueiredo, Carlos R. [UNIFESP]
dc.contributor.authorHosomi, Jorge K. [UNIFESP]
dc.contributor.authorNakamura, Mari U. [UNIFESP]
dc.contributor.authorLago, Joao Henrique G.
dc.coverageWesterville
dc.date.accessioned2020-07-08T13:09:42Z
dc.date.available2020-07-08T13:09:42Z
dc.date.issued2018
dc.description.abstractKalanchoe pinnata (Crassulaceae) is a medicinal plant distributed in several areas of the World. Their leaves have been used, in folk medicine, for anxiety and sleep disorders among other proposes. Chemically, this plant is composed by organic acids, flavonoids, bufadienolides, triterpenoids and some ubiquitous compounds. In this study, the fresh leaves of K. pinnata were extracted in an accelerated solvent extraction (ASE) system using H2O (cold and hot) as well as with EtOH:H2O 1:1. The obtained crude extracts were analyzed by HPLC-UV-MS. As a result, were identified organic acids and flavonoids, these being quercetin-3-O-alpha-L-arabinopyranosyl-(1 -> 2)-alpha-L-rhamnopyranoside, kaempferol-3-O-alpha-L-arabinopiranosyl-(1 -> 2)-alpha-L-rhamnopyranoside, quercitrin, and trans-p-coumaryl glutaric acid the main compounds in the studied extracts. Obtained results revealed the predominance of flavonoids in extracts prepared using hot H2O (70.26 +/- 2.19%) or EtOH:H2O 1:1 (74.65 +/- 5.39%) while a reduction in the amount of these compounds was observed in the extracts prepared using cold H2O (54.41 +/- 2.93%). Additionally, crude extracts from leaves of K. pinnata extracted with hot H2O and EtOH:H2O 1:1 showed in vitro cytotoxicity on five tested cancer cell lines: murine melanoma (B16F10), human melanoma (A2058), human cervical carcinoma (HeLa), human leukemia (HL-60), and human glioblastoma (U87-MG), with IC50 values in the range of 10.6 +/- 2.5 - 91.3 +/- 7.2 mu g/mL. On the other hand, the extract prepared using cold H2O displayed reduced potential. These crude extracts were also tested against human foreskin fibroblasts (non-tumorigenic cells) showing IC50 values ranging from 79.5 to 90.2 mu g/mL. In conclusion, our results showed the impact that different extraction methods have on the chemical composition and pharmacological activity, which are notable for the anticancer activity associated to flavonoids.en
dc.description.affiliationUniv Fed Sao Paulo, Dept Obstet, BR-04021001 Sao Paulo, SP, Brazil
dc.description.affiliationFed Univ ABC, Ctr Nat Sci & Humanities, BR-09210580 Santo Andre, SP, Brazil
dc.description.affiliationAnhanguera Univ Sao Paulo, Biotechnol & Innovat Hlth Postgrad Program, BR-05145200 Sao Paulo, SP, Brazil
dc.description.affiliationUniv Fed Sao Paulo, Dept Microbiol Immunol & Parasitol, BR-04023900 Sao Paulo, SP, Brazil
dc.description.affiliationUnifespUniv Fed Sao Paulo, Dept Obstet, BR-04021001 Sao Paulo, SP, Brazil
dc.description.affiliationUnifespUniv Fed Sao Paulo, Dept Microbiol Immunol & Parasitol, BR-04023900 Sao Paulo, SP, Brazil
dc.description.sourceWeb of Science
dc.description.sponsorshipFAPESP
dc.description.sponsorshipCNPq
dc.format.extent163-166
dc.identifierhttps://doi.org/10.1177/1934578X1801300213
dc.identifier.citationNatural Product Communications. Westerville, v. 13, n. 2, p. 163-166, 2018.
dc.identifier.doi10.1177/1934578X1801300213
dc.identifier.issn1934-578X
dc.identifier.urihttps://repositorio.unifesp.br/handle/11600/54148
dc.identifier.wosWOS:000433651700013
dc.language.isoeng
dc.publisherNatural Products Inc
dc.relation.ispartofNatural Product Communications
dc.rightsAcesso aberto
dc.subjectKalanchoe pinnataen
dc.subjectAccelerated solvent extraction (ASE)en
dc.subjectChemical compositionen
dc.subjectFlavonoidsen
dc.subjectAnticancer activityen
dc.titleChemical Composition and Cytotoxicity of Kalanchoe pinnata Leaves Extracts prepared using Accelerated System Extraction (ASE)en
dc.typeArtigo
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