Can measurement of the fluorescence lifetime of extracted blood PPIX predict atherosclerosis?

Can measurement of the fluorescence lifetime of extracted blood PPIX predict atherosclerosis?

Author Sicchieri, Leticia B. Google Scholar
Da Silva, Monica N. Autor UNIFESP Google Scholar
Samad, Ricardo E. Google Scholar
Courrol, Lilia C. Autor UNIFESP Google Scholar
Abstract In this work, fluorescence lifetime has been used to analyze protoporphyrin IX (PPIX) extracted from blood for diagnosing atherosclerosis. A total of 10 adult white male rabbits (New Zealand) were divided into the control group (CG), with a normal diet, and the experimental group (EG), subjected to a diet containing 1% cholesterol. Blood samples were collected from the animals, and protoporphyrin IX was extracted from the blood using acetone. The PPIX fluorescence lifetime (PPIXFL) was measured using time-correlated single photon counting, after excitation at 403 nm from a pulsed laser diode. It was found that the PPIX emission intensity was enhanced in the animals that had received a hypercholesterolemic diet. The CG and EG animal's fluorescence decays were fitted by three exponentials and the mean lifetimes were 4.0 ns and 9.5 ns, respectively. This lifetime dependence resulted in a calibration curve that allows the determination of the PPIX concentration with a temporal measurement. The obtained results show that fluorescence lifetime can potentially be used as a noninvasive, simple, rapid, and sensitive tool in atherosclerosis diagnosis.
Keywords Atherosclerosis
Protoporphyrin IX
Fluorescence lifetime
Blood analysis
xmlui.dri2xhtml.METS-1.0.item-coverage Amsterdam
Language English
Sponsor Fundacao de Amparo a Pesquisa do Estado de Sao Paulo (FAPESP)
Grant number FAPESP: 2014/06960-9
Date 2018
Published in Journal Of Luminescence. Amsterdam, v. 195, p. 176-180, 2018.
ISSN 0022-2313 (Sherpa/Romeo, impact factor)
Publisher Elsevier Science Bv
Extent 176-180
Access rights Open access Open Access
Type Article
Web of Science ID WOS:000418470500026

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