Can measurement of the fluorescence lifetime of extracted blood PPIX predict atherosclerosis?

Date
2018Author
Sicchieri, Leticia B.
Da Silva, Monica N. [UNIFESP]
Samad, Ricardo E.
Courrol, Lilia C. [UNIFESP]
Type
ArtigoISSN
0022-2313Is part of
Journal Of LuminescenceDOI
10.1016/j.jlumin.2017.11.014Metadata
Show full item recordAbstract
In this work, fluorescence lifetime has been used to analyze protoporphyrin IX (PPIX) extracted from blood for diagnosing atherosclerosis. A total of 10 adult white male rabbits (New Zealand) were divided into the control group (CG), with a normal diet, and the experimental group (EG), subjected to a diet containing 1% cholesterol. Blood samples were collected from the animals, and protoporphyrin IX was extracted from the blood using acetone. The PPIX fluorescence lifetime (PPIXFL) was measured using time-correlated single photon counting, after excitation at 403 nm from a pulsed laser diode. It was found that the PPIX emission intensity was enhanced in the animals that had received a hypercholesterolemic diet. The CG and EG animal's fluorescence decays were fitted by three exponentials and the mean lifetimes were 4.0 ns and 9.5 ns, respectively. This lifetime dependence resulted in a calibration curve that allows the determination of the PPIX concentration with a temporal measurement. The obtained results show that fluorescence lifetime can potentially be used as a noninvasive, simple, rapid, and sensitive tool in atherosclerosis diagnosis.
Citation
Journal Of Luminescence. Amsterdam, v. 195, p. 176-180, 2018.Sponsorship
Fundacao de Amparo a Pesquisa do Estado de Sao Paulo (FAPESP)Collections
- ICAQF - Artigos [1140]