Can measurement of the fluorescence lifetime of extracted blood PPIX predict atherosclerosis?

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dc.citation.volume195
dc.contributor.authorSicchieri, Leticia B.
dc.contributor.authorDa Silva, Monica N. [UNIFESP]
dc.contributor.authorSamad, Ricardo E.
dc.contributor.authorCourrol, Lilia C. [UNIFESP]
dc.coverageAmsterdam
dc.date.accessioned2020-07-08T13:09:37Z
dc.date.available2020-07-08T13:09:37Z
dc.date.issued2018
dc.description.abstractIn this work, fluorescence lifetime has been used to analyze protoporphyrin IX (PPIX) extracted from blood for diagnosing atherosclerosis. A total of 10 adult white male rabbits (New Zealand) were divided into the control group (CG), with a normal diet, and the experimental group (EG), subjected to a diet containing 1% cholesterol. Blood samples were collected from the animals, and protoporphyrin IX was extracted from the blood using acetone. The PPIX fluorescence lifetime (PPIXFL) was measured using time-correlated single photon counting, after excitation at 403 nm from a pulsed laser diode. It was found that the PPIX emission intensity was enhanced in the animals that had received a hypercholesterolemic diet. The CG and EG animal's fluorescence decays were fitted by three exponentials and the mean lifetimes were 4.0 ns and 9.5 ns, respectively. This lifetime dependence resulted in a calibration curve that allows the determination of the PPIX concentration with a temporal measurement. The obtained results show that fluorescence lifetime can potentially be used as a noninvasive, simple, rapid, and sensitive tool in atherosclerosis diagnosis.en
dc.description.affiliationIPEN CNEN SP, Sao Paulo, SP, Brazil
dc.description.affiliationUniv Fed Sao Paulo, Dept Fis, Diadema, SP, Brazil
dc.description.affiliationUnifespUniv Fed Sao Paulo, Dept Fis, Diadema, SP, Brazil
dc.description.sourceWeb of Science
dc.description.sponsorshipFundacao de Amparo a Pesquisa do Estado de Sao Paulo (FAPESP)
dc.description.sponsorshipIDFAPESP: 2014/06960-9
dc.format.extent176-180
dc.identifierhttp://dx.doi.org/10.1016/j.jlumin.2017.11.014
dc.identifier.citationJournal Of Luminescence. Amsterdam, v. 195, p. 176-180, 2018.
dc.identifier.doi10.1016/j.jlumin.2017.11.014
dc.identifier.issn0022-2313
dc.identifier.urihttps://repositorio.unifesp.br/handle/11600/54104
dc.identifier.wosWOS:000418470500026
dc.language.isoeng
dc.publisherElsevier Science Bv
dc.relation.ispartofJournal Of Luminescence
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subjectAtherosclerosisen
dc.subjectProtoporphyrin IXen
dc.subjectFluorescence lifetimeen
dc.subjectBlood analysisen
dc.titleCan measurement of the fluorescence lifetime of extracted blood PPIX predict atherosclerosis?en
dc.typeinfo:eu-repo/semantics/article
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