H-1-NMR, H-1-NMR T-2-edited, and 2D-NMR in bipolar disorder metabolic profiling

H-1-NMR, H-1-NMR T-2-edited, and 2D-NMR in bipolar disorder metabolic profiling

Author Sethi, Sumit Autor UNIFESP Google Scholar
Pedrini, Mariana Autor UNIFESP Google Scholar
Rizzo, Lucas B. Autor UNIFESP Google Scholar
Zeni-Graiff, Maiara Autor UNIFESP Google Scholar
Dal Mas, Caroline Autor UNIFESP Google Scholar
Cassinelli, Ana Claudia Google Scholar
Noto, Mariane N. Autor UNIFESP Google Scholar
Asevedo, Elson Autor UNIFESP Google Scholar
Cordeiro, Quirino Google Scholar
Pontes, Joao G. M. Google Scholar
Brasil, Antonio J. M. Google Scholar
Lacerda, Acioly Autor UNIFESP Google Scholar
Hayashi, Mirian A. F. Autor UNIFESP Google Scholar
Poppi, Ronei Google Scholar
Tasic, Ljubica Google Scholar
Brietzke, Elisa Autor UNIFESP Google Scholar
Abstract Background: The objective of this study was to identify molecular alterations in the human blood serum related to bipolar disorder, using nuclear magnetic resonance (NMR) spectroscopy and chemometrics. Methods: Metabolomic profiling, employing H-1-NMR, H-1-NMR -T-2-edited, and 2D-NMR spectroscopy and chemometrics of human blood serum samples from patients with bipolar disorder (n = 26) compared with healthy volunteers (n = 50) was performed. Results: The investigated groups presented distinct metabolic profiles, in which the main differential metabolites found in the serum sample of bipolar disorder patients compared with those from controls were lipids, lipid metabolism-related molecules (choline, myo-inositol), and some amino acids (N-acetyl-L-phenyl alanine, N-acetyl-L-aspartyl-L-glutamic acid, L-glutamine). In addition, amygdalin, alpha-ketoglutaric acid, and lipoamide, among other compounds, were also present or were significantly altered in the serum of bipolar disorder patients. The data presented herein suggest that some of these metabolites differentially distributed between the groups studied may be directly related to the bipolar disorder pathophysiology. Conclusions: The strategy employed here showed significant potential for exploring pathophysiological features and molecular pathways involved in bipolar disorder. Thus, our findings may contribute to pave the way for future studies aiming at identifying important potential biomarkers for bipolar disorder diagnosis or progression follow-up.
Keywords H-1-NMR
Biomarkers
Bipolar disorder
Metabolic profiling
Chemometrics
2D NMR
xmlui.dri2xhtml.METS-1.0.item-coverage Heidelberg
Language English
Sponsor Conselho Nacional de Desenvolvimento Cientifico e Tecnologico (CNPq, Brasilia, Brazil)
FAPESP
Grant number CNPq
FAPESP: 2014/18938-8
Date 2017
Published in International Journal Of Bipolar Disorders. Heidelberg, v. 5, p. -, 2017.
ISSN 2194-7511 (Sherpa/Romeo, impact factor)
Publisher Springer Heidelberg
Extent -
Origin http://dx.doi.org/10.1186/s40345-017-0088-2
Access rights ACESSO ABERTO
Type Article
Web of Science ID WOS:000403186100001
URI https://repositorio.unifesp.br/handle/11600/53670

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