Influência da compressão em fibroblastos do ligamento periodontal humano marcados com aldeído desidrogenase
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Data
2018-03-30
Autores
Miguel, Fabio Schemann [UNIFESP]
Orientadores
Ferreira, Lydia Masako [UNIFESP]
Tipo
Tese de doutorado
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Introdução: Os fibroblastos são as principais células do ligamento periodontal, responsáveis pela sua manutenção, regeneração e função. Fibroblastos do ligamento periodontal foram submetidos a diferentes cargas de pressão e posteriormente marcados por marcador tumoral o aldeído desidrogenase (ALDH) uma enzima que participa na oxidação intracelular de aldeídos e converte o retinol em ácido retinoico. Objetivo: Analisar fibroblastos marcados com ALDH derivados do ligamento periodontal humano em modelo de compressão estática contínua. Métodos: Foram selecionados dez pacientes, submetidos a extrações dos quatro terceiros molares inclusos por indicação ortodôntica. A amostra consistiu de 4 mm2 de tecido periodontal do terço médio das raízes. Os fibroblastos extraídos desse tecido foram cultivados até a sexta passagem e depois marcados com o marcador tumoral (ALDH) e separados em três grupos: Grupo Controle (GC), sem aplicação de carga durante 6 horas; Grupo Experimental 1 (GE1), com aplicação de carga de 3 g/mm2 durante o tempo de 6 horas; e Grupo Experimental 2 (GE2) com aplicação de carga de 4 g/mm2 durante o tempo de 6 horas. Para a identificação e quantificação, foi utilizado o kit comercial Aldefluor®. Resultados: Os grupos GE1 de 3 g/mm2 e GE2 de 4 g/mm2 apresentaram menor quantidade de células marcadas por ALDH na comparação com o grupo controle e o grupo GE2 de 4 g/mm2 apresentou maior quantidade de células marcadas, em relação ao grupo GE1 de 3 g/mm2, todos os resultados com relevância estatística. Conclusão: Os fibroblastos periodontais adultos marcados com ALDH diminuíram quando da aplicação de compressão estática contínua.
Background: Fibroblasts are the main cells of the periodontal ligament, responsible for their maintenance, regeneration and function. Periodontal ligament fibroblasts were subjected to different pressure loads and later labeled by tumor marker aldehyde dehydrogenase (ALDH), an enzyme that participates in the intracellular oxidation of aldehydes and converts retinol to retinoic acid. Fibroblasts are the main cells of the periodontal ligament, responsible for their maintenance, regeneration and function. Periodontal ligament fibroblasts were subjected to different pressure loads and later labeled by tumor marker aldehyde dehydrogenase (ALDH), an enzyme that participates in the intracellular oxidation of aldehydes and converts retinol to retinoic acid. Objective: To analyze ALDH-labeled fibroblasts derived from the human periodontal ligament in a continuous static compression model. Methods: Periodontal fibroblast isolated from 4 mm2 of periodontal tissue from third molars from ten patients. The cells were cultivated in culture media containing for six passages and then stained with ALDH. Cells from each donor were divided in three groups: Control Group (no “weight”); experimental Group 1 (EG1) (3 g/mm2 for 6 hours) and Experimental Group 2 (EG2) (4 g/mm2 for 6 hours). The Aldefluor® commercial kit was used for its identification and quantification. Results: The experimental groups showed significant reduction in the number of ALDH stained cells compared to the control group and the EG2 showed the higher number compared to EG1. Conclusion: Periodontal fibroblasts marked withALDH decreased when applying continuous static compression.
Background: Fibroblasts are the main cells of the periodontal ligament, responsible for their maintenance, regeneration and function. Periodontal ligament fibroblasts were subjected to different pressure loads and later labeled by tumor marker aldehyde dehydrogenase (ALDH), an enzyme that participates in the intracellular oxidation of aldehydes and converts retinol to retinoic acid. Fibroblasts are the main cells of the periodontal ligament, responsible for their maintenance, regeneration and function. Periodontal ligament fibroblasts were subjected to different pressure loads and later labeled by tumor marker aldehyde dehydrogenase (ALDH), an enzyme that participates in the intracellular oxidation of aldehydes and converts retinol to retinoic acid. Objective: To analyze ALDH-labeled fibroblasts derived from the human periodontal ligament in a continuous static compression model. Methods: Periodontal fibroblast isolated from 4 mm2 of periodontal tissue from third molars from ten patients. The cells were cultivated in culture media containing for six passages and then stained with ALDH. Cells from each donor were divided in three groups: Control Group (no “weight”); experimental Group 1 (EG1) (3 g/mm2 for 6 hours) and Experimental Group 2 (EG2) (4 g/mm2 for 6 hours). The Aldefluor® commercial kit was used for its identification and quantification. Results: The experimental groups showed significant reduction in the number of ALDH stained cells compared to the control group and the EG2 showed the higher number compared to EG1. Conclusion: Periodontal fibroblasts marked withALDH decreased when applying continuous static compression.
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MIGUEL, Fabio Schemann. Influência da compressão em fibroblastos do ligamento periodontal humano marcados com aldeído desidrogenase. 2018. 118 f. Tese (Doutorado em Cirurgia Translacional) - Escola Paulista de Medicina, Universidade Federal de São Paulo, São Paulo, 2018.