Avaliação da evolução do tumor pulmonar experimental induzido por uretana em camundongos sedentários Santos
Arquivos
Data
2013-02-18
Autores
Tomás, Caio Marins 
Orientadores
Bueno, Valquiria
Tipo
Trabalho de conclusão de curso
Título da Revista
ISSN da Revista
Título de Volume
Resumo
Tumores “escapam” da função antitumoral pela secreção de fatores que inibem o
sistema imune. Um efeito dos fatores secretados pelo tumor é o aumento das células
mieloides supressoras (MDSC) que suprimem a resposta imune favorecendo assim o
desenvolvimento tumoral. Buscando compreender a ação do câncer sobre as células do
sistema imune, nosso grupo avaliou as mudanças quantitativas das células da resposta
imune (linfócitos e mielóides supressoras) em camundongos BALB/c sedentários com
câncer de pulmão induzido por um carcinógeno químico. Os camundongos foram
induzidos ao câncer de pulmão pela administração de Uretana (120 dias para formação
de nódulos pulmonares) e após o desenvolvimento tumoral o baço, timo, medula óssea,
e o sangue foram coletados e avaliados por citometria de fluxo (T CD4, T CD8, além
das MDSC - CD11b+Gr-1+). Os pulmões foram removidos para análise histológica
(número e área dos nódulos tumorais). Nosso intuito foi comparar camundongos
sedentários saudáveis e sedentários com câncer de pulmão induzido por Uretana quanto
á quantidade de linfócitos e células mielóides supressoras em alguns órgãos linfóides
(timo, medula óssea, baço) e sangue. Também avaliamos, se o possível aumento de
células mielóides supressoras esteve relacionado ao aumento da área e número de
nódulos pulmonares. Os resultados contribuirão para o melhor entendimento do câncer
de pulmão e sua relação com a expressão de MDSC em camundongos BALB/c
sedentários. Na sequência é meu interesse propor modalidades de exercício físico que
possam agir sobre as células do sistema imune como terapia de restrição do
desenvolvimento tumoral.
Tumors "escape" from the antitumor function by secreting factors that inhibit the immune system. Factors secreted by tumors affect the immune system by inducing the increase of myeloid derived suppressor cells (MDSC) which suppress the immune response and thus promote tumor development. In order to further understand how cancer acts on immune system cells, we assessed the quantitative changes of immune response cells (lymphocytes and myeloid suppressor) in sedentary BALB/c mice with lung cancer induced by a chemical carcinogen (Urethane). Mice were injected with Urethane with the aim to induce lung cancer (120 days for lung nodule formation) and after the tumor development the spleen, thymus, bone marrow and the blood were collected and assessed by flow cytometry (T CD4, T CD8, besides of MDSC -CD11b+ Gr-1+). Lungs were removed to histological analysis (number and area of neoplastic nodules). Our aim was to quantify lymphocytes and myeloid suppressor cells in some lymphoid organs (thymus, bone marrow, spleen) and blood from sedentary healthy mice and sedentary urethane-induced lung cancer. We also evaluated whether a possible increase of myeloid suppressor cells was related to the increase in area and number of lung nodules. The results will contribute to the better understanding of lung cancer and its relationship with the expression of MDSC in sedentary BALB/c mice. Our future interest is to propose methods of exercise that may act on the immune system cells as therapy of tumour development constraint
Tumors "escape" from the antitumor function by secreting factors that inhibit the immune system. Factors secreted by tumors affect the immune system by inducing the increase of myeloid derived suppressor cells (MDSC) which suppress the immune response and thus promote tumor development. In order to further understand how cancer acts on immune system cells, we assessed the quantitative changes of immune response cells (lymphocytes and myeloid suppressor) in sedentary BALB/c mice with lung cancer induced by a chemical carcinogen (Urethane). Mice were injected with Urethane with the aim to induce lung cancer (120 days for lung nodule formation) and after the tumor development the spleen, thymus, bone marrow and the blood were collected and assessed by flow cytometry (T CD4, T CD8, besides of MDSC -CD11b+ Gr-1+). Lungs were removed to histological analysis (number and area of neoplastic nodules). Our aim was to quantify lymphocytes and myeloid suppressor cells in some lymphoid organs (thymus, bone marrow, spleen) and blood from sedentary healthy mice and sedentary urethane-induced lung cancer. We also evaluated whether a possible increase of myeloid suppressor cells was related to the increase in area and number of lung nodules. The results will contribute to the better understanding of lung cancer and its relationship with the expression of MDSC in sedentary BALB/c mice. Our future interest is to propose methods of exercise that may act on the immune system cells as therapy of tumour development constraint
Descrição
Citação
TOMÁS, Caio Marins.Avaliação da evolução do tumor pulmonar experimental induzido por uretana em camundongos sedentários Santos. 2012. 38 f.Trabalho de conclusão de curso de graduação (Educação Física) - Instituto de Saúde e Sociedade, Universidade Federal de São Paulo, Santos, 2013.