Interação entre fatores oceanográficos, expressão de proteínas e a atividade de proteases em Stramonita brasiliensis
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Date
2019-10-30
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Britto, Gustavo Gesini [UNIFESP]
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Christofoletti, Ronaldo Adriano [UNIFESP]
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Dissertação de mestrado
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Abstract
Os costões rochosos são ambientes dinâmicos, onde fatores oceanográficos mudam combinados ao ciclo das marés. Os organismos habitantes desses costões rochosos, como a Stramonita sp., possuem adaptações para lidar com a pressão ambiental, como mudanças na expressão de proteínas, capazes de mudar o seu metabolismo em situações de estresse. Entre essas proteínas estão as proteases, enzimas que participam de processos fundamentais para o crescimento e sobrevivência dos organismos. Neste estudo interdisciplinar, buscamos verificar a relação entre fatores oceanográficos, a expressão de proteínas e a atividade de proteases em Stramonita brasiliensis. Foram coletados organismos e dados ambientais, como a exposição às ondas, temperatura superficial da água (TSM) e produtividade primária, em 13 costões rochosos do litoral sul de São Paulo ao norte do Rio de Janeiro. Foram escolhidos 5 organismos de cada costão rochoso de acordo com sua massa sem a concha, os quais foram avaliados quanto a expressão de proteínas e a atividade de proteases. Nos organismos desses costões rochosos, a expressão de proteínas teve uma relação inversa com a atividade de proteases. As variáveis oceanográficas influenciaram tanto a expressão de proteínas como a atividade de proteases. Em áreas mais quentes (TSM maior do que 25ºC) foram observadas as maiores concentrações de proteínas, mas nas áreas mais frias (TSM menor do que 25ºC) foram observadas as maiores atividades enzimáticas de proteases. Esses resultados mostram que os fatores oceanográticos influenciam na expressão de proteínas e na atividade de proteases de Stramonita brasiliensis e consistem em um avanço para a compreensão das respostas fisiológicas que os invertebrados marinhos possuem em diferentes condições ambientais
Rocky shores are dynamic environments where oceanographic factors change combined with the tidal cycle. The organisms inhabiting these rocky shores, such as Stramonita sp., Have adaptations to cope with environmental pressure, such as changes in protein expression, capable of changing their metabolism under stress. Among these proteins are proteases, enzymes that participate in fundamental processes for the growth and survival of organisms. In this interdisciplinary study, we sought to verify the relationship between oceanographic factors, protein expression and protease activity in Stramonita brasiliensis. Environmental organisms and data such as wave exposure, surface water temperature (SST) and primary productivity (Chl-a) were collected from 13 rocky shores of the south coast of São Paulo north of Rio de Janeiro. Five organisms from each rocky shore were chosen according to their mass without the shell, which were evaluated for protein expression and protease activity. In organisms on these rocky shores, protein expression has an inverse relationship to protease activity. Oceanographic variables influenced both protein expression and protease activity. Protein expression was higher in organisms from areas with SST below 25 °C (cold) than in areas with SST above 25 °C (warm). In warmer areas the highest protein concentrations were observed, but in the colder areas the highest protease enzymatic activities in general were observed. These results show that oceanographic factors influence protein expression and protease activity of Stramonita brasiliensis and constitute an advance for the understanding of the physiological responses that marine invertebrates have in different environmental conditions
Rocky shores are dynamic environments where oceanographic factors change combined with the tidal cycle. The organisms inhabiting these rocky shores, such as Stramonita sp., Have adaptations to cope with environmental pressure, such as changes in protein expression, capable of changing their metabolism under stress. Among these proteins are proteases, enzymes that participate in fundamental processes for the growth and survival of organisms. In this interdisciplinary study, we sought to verify the relationship between oceanographic factors, protein expression and protease activity in Stramonita brasiliensis. Environmental organisms and data such as wave exposure, surface water temperature (SST) and primary productivity (Chl-a) were collected from 13 rocky shores of the south coast of São Paulo north of Rio de Janeiro. Five organisms from each rocky shore were chosen according to their mass without the shell, which were evaluated for protein expression and protease activity. In organisms on these rocky shores, protein expression has an inverse relationship to protease activity. Oceanographic variables influenced both protein expression and protease activity. Protein expression was higher in organisms from areas with SST below 25 °C (cold) than in areas with SST above 25 °C (warm). In warmer areas the highest protein concentrations were observed, but in the colder areas the highest protease enzymatic activities in general were observed. These results show that oceanographic factors influence protein expression and protease activity of Stramonita brasiliensis and constitute an advance for the understanding of the physiological responses that marine invertebrates have in different environmental conditions
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BRITTO, Gustavo Gesini. Interação entre fatores oceanográficos, expressão de proteínas e a atividade de proteases em Stramonita brasiliensis. 2019. 67 f. Dissertação (Mestrado em Ciências) - Instituto do Mar, Universidade Federal de São Paulo, Santos, 2019.