Quercetin-modulated erythrocyte membrane sodium-hydrogen exchanger during human aging: correlation with atpase's
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2016
Authors
Kumar, Prabhanshu
Chand, Subhash
Maurya, Pawan Kumar [UNIFESP]
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Abstract
Content: Quercetin uptake by erythrocytes is rapid. The sodium-hydrogen exchanger (NHE) is a secondary active transporter, regulating intracellular pH, Na+ concentration and cell volume. Objective: The aim of present study was to investigate NHE as a function of human age and effect of quercetin on its activity. The NHE activity was correlated with erythrocytes ATPases. Materials and methods: We analyzed normal, healthy subjects of both sexes (20-82 years). NHE activity was estimated in terms of amiloride-sensitive H+-efflux from acid-loaded cells. Results: A significant age-dependent increase in NHE activity was observed during aging in humans. Concentration (10(-3) M to 10(-8) M)-dependent in vitro treatment with quercetin causes inhibition of NHE activity. The Na+/K+ -ATPase (r = 0.8882) and Ca2+-ATPase (r = 0.9540) activities positively correlated with it. Discussion and conclusion: The present data show an additional mechanism where dietary flavonoids may exerts beneficial effect during aging.
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Archives Of Physiology And Biochemistry. Abingdon, v. 122, n. 3, p. 141-147, 2016.