CRF type 1 receptors of the medial amygdala modulate inhibitory avoidance responses in the elevated T-maze

CRF type 1 receptors of the medial amygdala modulate inhibitory avoidance responses in the elevated T-maze

Author Vicentini, Jéssica Elias Autor UNIFESP Google Scholar
Céspedes, Isabel Cristina Autor UNIFESP Google Scholar
Nascimento, Juliana Olivetti Guzman Autor UNIFESP Google Scholar
Bittencourt, Jackson C. Google Scholar
Viana, Milena de Barros Autor UNIFESP Google Scholar
Institution Universidade Federal de São Paulo (UNIFESP)
Universidade de São Paulo (USP)
Abstract Corticotropin-releasing factor (CRF) plays a critical role in the mediation of physiological and behavioral responses to stressors. in the present study, we investigated the role played by the CRF system within the medial amygdala (MeA) in the modulation of anxiety and fear-related responses. Male Wistar rats were bilaterally administered into the MeA with CRF (125 and 250 ng/0.2 mu l, experiment 1) or with the CRFR1 antagonist antalarmin (25 ng/0.2 mu l, experiment 2) and 10 min later tested in the elevated T-maze (ETM) for inhibitory avoidance and escape measurements. in clinical terms, these responses have been respectively related to generalized anxiety and panic disorder. To further verify if the anxiogenic effects of CRF were mediated by CRFR1 activation, we also investigated the effects of the combined treatment with CRF (250 ng/0.2 mu l) and antalarmin (25 ng/0.2 mu l) (experiment 3). All animals were tested in an open field, immediately after the ETM, for locomotor activity assessment. Results showed that CRF, in the two doses administered, facilitated ETM avoidance, an anxiogenic response. Antalarmin significantly decreased avoidance latencies, an anxiolytic effect, and was able to counteract the anxiogenic effects of CRF. None of the compounds administered altered escape responses or locomotor activity measurements. These results suggest that CRF in the MeA exerts anxiogenic effects by activating type 1 receptors, which might be of relevance to the physiopathology of generalized anxiety disorder. (C) 2014 Elsevier Inc. All rights reserved.
Keywords Corticotropin-releasing factor CRF
Medial amygdala
Elevated T-maze
Language English
Sponsor Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
Date 2014-03-01
Published in Hormones and Behavior. San Diego: Academic Press Inc Elsevier Science, v. 65, n. 3, p. 195-202, 2014.
ISSN 0018-506X (Sherpa/Romeo, impact factor)
Publisher Elsevier B.V.
Extent 195-202
Access rights Closed access
Type Article
Web of Science ID WOS:000333228900001

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