CRF type 1 receptors of the medial amygdala modulate inhibitory avoidance responses in the elevated T-maze

dc.contributor.authorVicentini, Jéssica Elias [UNIFESP]
dc.contributor.authorCéspedes, Isabel Cristina [UNIFESP]
dc.contributor.authorNascimento, Juliana Olivetti Guzman [UNIFESP]
dc.contributor.authorBittencourt, Jackson C.
dc.contributor.authorViana, Milena de Barros [UNIFESP]
dc.contributor.institutionUniversidade Federal de São Paulo (UNIFESP)
dc.contributor.institutionUniversidade de São Paulo (USP)
dc.date.accessioned2016-01-24T14:35:23Z
dc.date.available2016-01-24T14:35:23Z
dc.date.issued2014-03-01
dc.description.abstractCorticotropin-releasing factor (CRF) plays a critical role in the mediation of physiological and behavioral responses to stressors. in the present study, we investigated the role played by the CRF system within the medial amygdala (MeA) in the modulation of anxiety and fear-related responses. Male Wistar rats were bilaterally administered into the MeA with CRF (125 and 250 ng/0.2 mu l, experiment 1) or with the CRFR1 antagonist antalarmin (25 ng/0.2 mu l, experiment 2) and 10 min later tested in the elevated T-maze (ETM) for inhibitory avoidance and escape measurements. in clinical terms, these responses have been respectively related to generalized anxiety and panic disorder. To further verify if the anxiogenic effects of CRF were mediated by CRFR1 activation, we also investigated the effects of the combined treatment with CRF (250 ng/0.2 mu l) and antalarmin (25 ng/0.2 mu l) (experiment 3). All animals were tested in an open field, immediately after the ETM, for locomotor activity assessment. Results showed that CRF, in the two doses administered, facilitated ETM avoidance, an anxiogenic response. Antalarmin significantly decreased avoidance latencies, an anxiolytic effect, and was able to counteract the anxiogenic effects of CRF. None of the compounds administered altered escape responses or locomotor activity measurements. These results suggest that CRF in the MeA exerts anxiogenic effects by activating type 1 receptors, which might be of relevance to the physiopathology of generalized anxiety disorder. (C) 2014 Elsevier Inc. All rights reserved.en
dc.description.affiliationUniversidade Federal de São Paulo, Dept Biociencias, BR-11060001 Santos, Brazil
dc.description.affiliationUniversidade Federal de São Paulo, Dept Psiquiatria & Psicol Med, BR-04038020 São Paulo, Brazil
dc.description.affiliationUniv São Paulo, Dept Anat, Inst Ciencias Biomed, BR-05508000 São Paulo, Brazil
dc.description.affiliationUnifespUniversidade Federal de São Paulo, Dept Biociencias, BR-11060001 Santos, Brazil
dc.description.affiliationUnifespUniversidade Federal de São Paulo, Dept Psiquiatria & Psicol Med, BR-04038020 São Paulo, Brazil
dc.description.sourceWeb of Science
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.description.sponsorshipCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
dc.format.extent195-202
dc.identifierhttps://dx.doi.org/10.1016/j.yhbeh.2014.01.004
dc.identifier.citationHormones and Behavior. San Diego: Academic Press Inc Elsevier Science, v. 65, n. 3, p. 195-202, 2014.
dc.identifier.doi10.1016/j.yhbeh.2014.01.004
dc.identifier.issn0018-506X
dc.identifier.urihttps://repositorio.unifesp.br/handle/11600/37504
dc.identifier.wosWOS:000333228900001
dc.language.isoeng
dc.publisherElsevier B.V.
dc.relation.ispartofHormones and Behavior
dc.rightsinfo:eu-repo/semantics/restrictedAccess
dc.rights.licensehttp://www.elsevier.com/about/open-access/open-access-policies/article-posting-policy
dc.subjectCorticotropin-releasing factor CRFen
dc.subjectMedial amygdalaen
dc.subjectElevated T-mazeen
dc.subjectAnxietyen
dc.subjectFearen
dc.subjectPanicen
dc.titleCRF type 1 receptors of the medial amygdala modulate inhibitory avoidance responses in the elevated T-mazeen
dc.typeinfo:eu-repo/semantics/article
Arquivos
Coleções