Expression of genes encoding smooth muscle contractile proteins in vaginal tissue of women with and without pelvic organ prolapse

Date
2012-01-01Author
Bortolini, Maria A. T. [UNIFESP]
Shynlova, Oksana
Drutz, Harold P.
Castro, Rodrigo A. [UNIFESP]
Girao, Manoel J. B. C. [UNIFESP]
Lye, Stephen
Alarab, May
Type
ArtigoISSN
0733-2467Is part of
Neurourology and UrodynamicsDOI
10.1002/nau.21175Metadata
Show full item recordAbstract
Aims We hypothesize that the expression of genes encoding vaginal smooth muscle (SM) contractile proteins is altered in patients with pelvic organ prolapse (POP) and is influenced by age and menopausal status. We aim to analyze the expression of SM-myosin heavy chain (MHY11), caldesmon (CALD1), SM gamma-actin (ACTG2), and tropomyosin (TPM1), in premenopausal and postmenopausal women with advanced POP and asymptomatic controls. Methods: During total hysterectomy we collected anterior vaginal wall biopsy samples from 55 women, 37 premenopausal (23 patients and 14 controls), and 18 postmenopausal women (13 patients and 5 controls). Total mRNA from the tissues was quantified by real-time RT-PCR. Results: MHY11 gene expression was down-regulated in premenopausal POP patients compared to premenopausal controls (fivefold, P = 0.002). in the postmenopausal groups, we observed a sixfold increase in the CALD1 gene expression in POP patients compared to asymptomatic controls (P = 0.03). the gene expression of CALD1, ACTG2, and TPM1 was significantly down-regulated in vaginal tissue of healthy women after menopause (P < 0.05). Conclusion: Dysregulation of the vaginal SM content in POP patients involves alteration of different cellular pathways according to age and menopausal status. Neurourol. Urodynam. 31:109-114, 2012. (C) 2011 Wiley Periodicals, Inc.
Citation
Neurourology and Urodynamics. Malden: Wiley-Blackwell, v. 31, n. 1, p. 109-114, 2012.Sponsorship
Physician's Services Inc. Foundation (PSI), Toronto, Ontario, CanadaCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
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