Expression of genes encoding smooth muscle contractile proteins in vaginal tissue of women with and without pelvic organ prolapse

Página do item simplificado
dc.contributor.authorBortolini, Maria Augusta Tezelli [UNIFESP]
dc.contributor.authorShynlova, Oksana
dc.contributor.authorDrutz, Harold P.
dc.contributor.authorCastro, Rodrigo de Aquino [UNIFESP]
dc.contributor.authorGirão, Manoel João Batista Castello [UNIFESP]
dc.contributor.authorLye, Stephen
dc.contributor.authorAlarab, May
dc.contributor.institutionUniv Toronto
dc.contributor.institutionUniversidade Federal de São Paulo (UNIFESP)
dc.contributor.institutionMt Sinai Hosp
dc.date.accessioned2016-01-24T14:17:41Z
dc.date.available2016-01-24T14:17:41Z
dc.date.issued2012-01-01
dc.description.abstractAims We hypothesize that the expression of genes encoding vaginal smooth muscle (SM) contractile proteins is altered in patients with pelvic organ prolapse (POP) and is influenced by age and menopausal status. We aim to analyze the expression of SM-myosin heavy chain (MHY11), caldesmon (CALD1), SM gamma-actin (ACTG2), and tropomyosin (TPM1), in premenopausal and postmenopausal women with advanced POP and asymptomatic controls. Methods: During total hysterectomy we collected anterior vaginal wall biopsy samples from 55 women, 37 premenopausal (23 patients and 14 controls), and 18 postmenopausal women (13 patients and 5 controls). Total mRNA from the tissues was quantified by real-time RT-PCR. Results: MHY11 gene expression was down-regulated in premenopausal POP patients compared to premenopausal controls (fivefold, P = 0.002). in the postmenopausal groups, we observed a sixfold increase in the CALD1 gene expression in POP patients compared to asymptomatic controls (P = 0.03). the gene expression of CALD1, ACTG2, and TPM1 was significantly down-regulated in vaginal tissue of healthy women after menopause (P < 0.05). Conclusion: Dysregulation of the vaginal SM content in POP patients involves alteration of different cellular pathways according to age and menopausal status. Neurourol. Urodynam. 31:109-114, 2012. (C) 2011 Wiley Periodicals, Inc.en
dc.description.affiliationUniv Toronto, Mt Sinai Hosp, Div Urogynecol & Reconstruct Pelv Surg, Dept Obstet & Gynecol, Toronto, ON M5G 1Z5, Canada
dc.description.affiliationUniversidade Federal de São Paulo, Dept Gynecol, Div Urogynecol & Reconstruct Pelv Surg, São Paulo, Brazil
dc.description.affiliationMt Sinai Hosp, Samuel Lunenfeld Res Inst, Toronto, ON M5G 1X5, Canada
dc.description.affiliationUnifespUniversidade Federal de São Paulo, Dept Gynecol, Div Urogynecol & Reconstruct Pelv Surg, São Paulo, Brazil
dc.description.sourceWeb of Science
dc.description.sponsorshipPhysician's Services Inc. Foundation (PSI), Toronto, Ontario, Canada
dc.description.sponsorshipCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
dc.format.extent109-114
dc.identifierhttps://dx.doi.org/10.1002/nau.21175
dc.identifier.citationNeurourology and Urodynamics. Malden: Wiley-Blackwell, v. 31, n. 1, p. 109-114, 2012.
dc.identifier.doi10.1002/nau.21175
dc.identifier.issn0733-2467
dc.identifier.urihttps://repositorio.unifesp.br/handle/11600/34433
dc.identifier.wosWOS:000299372300020
dc.language.isoeng
dc.publisherWiley-Blackwell
dc.relation.ispartofNeurourology and Urodynamics
dc.rightsinfo:eu-repo/semantics/restrictedAccess
dc.rights.licensehttp://olabout.wiley.com/WileyCDA/Section/id-406071.html
dc.subjectActinen
dc.subjectMenopauseen
dc.subjectMyosinen
dc.subjectPOPen
dc.subjectSmooth muscleen
dc.subjectVaginaen
dc.titleExpression of genes encoding smooth muscle contractile proteins in vaginal tissue of women with and without pelvic organ prolapseen
dc.typeinfo:eu-repo/semantics/article
Arquivos
Coleções
EPM - Artigos