Exhaustive Exercise Increases Inflammatory Response via Toll Like Receptor-4 and NF-kappa Bp65 Pathway in Rat Adipose Tissue

Exhaustive Exercise Increases Inflammatory Response via Toll Like Receptor-4 and NF-kappa Bp65 Pathway in Rat Adipose Tissue

Author Rosa Neto, José Cesar Autor UNIFESP Google Scholar
Lira, Fabio Santos de Autor UNIFESP Google Scholar
Eguchi, Ricardo Autor UNIFESP Google Scholar
Pimentel, Gustavo Duarte Autor UNIFESP Google Scholar
Venancio, Daniel Paulino Autor UNIFESP Google Scholar
Cunha, Claudio Alexandre Autor UNIFESP Google Scholar
Oyama, Lila Missae Autor UNIFESP Google Scholar
Mello, Marco Tulio de Autor UNIFESP Google Scholar
Seelaender, Marilia Google Scholar
Nascimento, Claudia Maria da Penha Oller do Autor UNIFESP Google Scholar
Institution Universidade Federal de São Paulo (UNIFESP)
Universidade de São Paulo (USP)
Abstract Cytokines (IL-6, IL-10, and TNF-alpha) are increased after exhaustive exercise in the retroperitoneal adipose tissue (RPAT) and mesenteric adipose tissue (MEAT). An exhaustive acute exercise protocol induces inflammation in adipose tissue that lasts 6 h after the exercise has ended. It is well-established that this protocol increases circulating plasma levels of non-esterified fatty acids (NEFAs) and lipopolysaccharides (LPS), compounds that are important in stimulating signaling via toll like receptor-4 (TLR-4) in different type cells. in the present study, we investigated the regulation of TLR-4 and DNA-binding of nuclear factor-kappa Bp65 (NF-kappa Bp65) in different depots of adipose tissue in rats after exhaustive exercise. Rats were killed by decapitation immediately (E0 group, n = 6), 2 (E2 group, n = 6), and 6 h (E6 group, n = 6) after the exhaustive exercise, which consisted of running on a treadmill (approximately 70% V-O2max) for 50 min and then running at an elevated rate that increased at 1 m/min, until exhaustion. the control group (C group, n = 6) was not subjected to exercise. in RPAT, TLR-4, MYD-88, and IkB alpha increased in the E2 group after exercise. MYD-88 and TRAF6 remained increased in the E6 group in comparison with the control group. DNA-binding of NF-kappa Bp65 was not altered. in MEAT, TLR-4, MYD-88, TRAF6, and DNA-binding of NF-kappa Bp65 were increased only in the E6 group. in conclusion, we have shown that increases in pro-inflammatory cytokines in adipose tissue pads after exhaustive exercise may be mediated via TLR-4 signaling, leading to increases in NF-kappa Bp65 binding to DNA in MEAT. J. Cell. Physiol. 226: 1604-1607, 2011. (C) 2010 Wiley-Liss, Inc.
Language English
Sponsor Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
Grant number FAPESP: 04/11588-0
CAPES: 05/01445-0
Date 2011-06-01
Published in Journal of Cellular Physiology. Hoboken: Wiley-Blackwell, v. 226, n. 6, p. 1604-1607, 2011.
ISSN 0021-9541 (Sherpa/Romeo, impact factor)
Publisher Wiley-Blackwell
Extent 1604-1607
Origin https://dx.doi.org/10.1002/jcp.22490
Access rights Closed access
Type Article
Web of Science ID WOS:000288610300020
URI https://repositorio.unifesp.br/handle/11600/33734

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