Exhaustive Exercise Increases Inflammatory Response via Toll Like Receptor-4 and NF-kappa Bp65 Pathway in Rat Adipose Tissue

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dc.contributor.authorRosa Neto, José Cesar [UNIFESP]
dc.contributor.authorLira, Fabio Santos de [UNIFESP]
dc.contributor.authorEguchi, Ricardo [UNIFESP]
dc.contributor.authorPimentel, Gustavo Duarte [UNIFESP]
dc.contributor.authorVenancio, Daniel Paulino [UNIFESP]
dc.contributor.authorCunha, Claudio Alexandre [UNIFESP]
dc.contributor.authorOyama, Lila Missae [UNIFESP]
dc.contributor.authorMello, Marco Tulio de [UNIFESP]
dc.contributor.authorSeelaender, Marilia
dc.contributor.authorNascimento, Claudia Maria da Penha Oller do [UNIFESP]
dc.contributor.institutionUniversidade Federal de São Paulo (UNIFESP)
dc.contributor.institutionUniversidade de São Paulo (USP)
dc.date.accessioned2016-01-24T14:16:48Z
dc.date.available2016-01-24T14:16:48Z
dc.date.issued2011-06-01
dc.description.abstractCytokines (IL-6, IL-10, and TNF-alpha) are increased after exhaustive exercise in the retroperitoneal adipose tissue (RPAT) and mesenteric adipose tissue (MEAT). An exhaustive acute exercise protocol induces inflammation in adipose tissue that lasts 6 h after the exercise has ended. It is well-established that this protocol increases circulating plasma levels of non-esterified fatty acids (NEFAs) and lipopolysaccharides (LPS), compounds that are important in stimulating signaling via toll like receptor-4 (TLR-4) in different type cells. in the present study, we investigated the regulation of TLR-4 and DNA-binding of nuclear factor-kappa Bp65 (NF-kappa Bp65) in different depots of adipose tissue in rats after exhaustive exercise. Rats were killed by decapitation immediately (E0 group, n = 6), 2 (E2 group, n = 6), and 6 h (E6 group, n = 6) after the exhaustive exercise, which consisted of running on a treadmill (approximately 70% V-O2max) for 50 min and then running at an elevated rate that increased at 1 m/min, until exhaustion. the control group (C group, n = 6) was not subjected to exercise. in RPAT, TLR-4, MYD-88, and IkB alpha increased in the E2 group after exercise. MYD-88 and TRAF6 remained increased in the E6 group in comparison with the control group. DNA-binding of NF-kappa Bp65 was not altered. in MEAT, TLR-4, MYD-88, TRAF6, and DNA-binding of NF-kappa Bp65 were increased only in the E6 group. in conclusion, we have shown that increases in pro-inflammatory cytokines in adipose tissue pads after exhaustive exercise may be mediated via TLR-4 signaling, leading to increases in NF-kappa Bp65 binding to DNA in MEAT. J. Cell. Physiol. 226: 1604-1607, 2011. (C) 2010 Wiley-Liss, Inc.en
dc.description.affiliationUniversidade Federal de São Paulo, Dept Physiol Nutr, BR-04023060 São Paulo, Brazil
dc.description.affiliationUniversidade Federal de São Paulo, Dept Psychobiol, BR-04023060 São Paulo, Brazil
dc.description.affiliationUniv São Paulo, Dept Cell & Dev Biol, Canc Metab Res Grp, Inst Biomed Sci 1, São Paulo, Brazil
dc.description.affiliationUnifespUniversidade Federal de São Paulo, Dept Physiol Nutr, BR-04023060 São Paulo, Brazil
dc.description.affiliationUnifespUniversidade Federal de São Paulo, Dept Psychobiol, BR-04023060 São Paulo, Brazil
dc.description.sourceWeb of Science
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.description.sponsorshipCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
dc.description.sponsorshipIDFAPESP: 04/11588-0
dc.description.sponsorshipIDCAPES: 05/01445-0
dc.format.extent1604-1607
dc.identifierhttps://dx.doi.org/10.1002/jcp.22490
dc.identifier.citationJournal of Cellular Physiology. Hoboken: Wiley-Blackwell, v. 226, n. 6, p. 1604-1607, 2011.
dc.identifier.doi10.1002/jcp.22490
dc.identifier.issn0021-9541
dc.identifier.urihttps://repositorio.unifesp.br/handle/11600/33734
dc.identifier.wosWOS:000288610300020
dc.language.isoeng
dc.publisherWiley-Blackwell
dc.relation.ispartofJournal of Cellular Physiology
dc.rightsinfo:eu-repo/semantics/restrictedAccess
dc.rights.licensehttp://olabout.wiley.com/WileyCDA/Section/id-406071.html
dc.titleExhaustive Exercise Increases Inflammatory Response via Toll Like Receptor-4 and NF-kappa Bp65 Pathway in Rat Adipose Tissueen
dc.typeinfo:eu-repo/semantics/article
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