Effects of melatonin on histomorphology and on the expression of steroid receptors, VEGF, and PCNA in ovaries of pinealectomized female rats

Effects of melatonin on histomorphology and on the expression of steroid receptors, VEGF, and PCNA in ovaries of pinealectomized female rats

Author Romeu, Lucrecia Regina Gomes Autor UNIFESP Google Scholar
Motta, Eduardo Leme Alves da Autor UNIFESP Google Scholar
Maganhin, Carla Cristina Autor UNIFESP Google Scholar
Oshima, Celina Tizuko Fujiyama Autor UNIFESP Google Scholar
Fonseca, Marcelle Cavalcante Autor UNIFESP Google Scholar
Barrueco, Karina Fonseca Autor UNIFESP Google Scholar
Simões, Ricardo Santos Autor UNIFESP Google Scholar
Pellegrino, Renata Autor UNIFESP Google Scholar
Baracat, Edmund Chada Autor UNIFESP Google Scholar
Soares Júnior, José Maria Autor UNIFESP Google Scholar
Institution Universidade Federal de São Paulo (UNIFESP)
Abstract Objective: To evaluate the effect of melatonin both on the ovaries of pinealectomized female rats through histomorphometric analysis and on steroid receptors, proliferating cell nuclear antigen (PCNA), and vascular endothelial growth factor (VEGF) expression.Design: Experimental study.Setting: Federal University of São Paulo, Brazil.Animal(s): Forty female rats.Intervention(s): Forty rats were divided equally into four groups: GI-vehicle without surgery; GII-surgery without removal of the pineal gland (sham); GIII-pinealectomized with vehicle; and GIV-pinealectomized with melatonin treatment. After treatment for 3 consecutive months, the animals were killed and their ovaries removed for analysis.Main Outcome Measure(s): Estrogen and progesterone receptors, histologic and immunohistochemical analysis.Result(s): the GIII samples presented signals of proliferation on ovarian surface epithelium and interstitial cells as well as high expressions of PCNA and VEGF in those structures compared with GI, GII, and GIV. Also, the levels of progesterone receptor (fmol/g) in ovaries of GIII (250.6 +/- 32.4) were significantly lower than in those of GI (429.0 +/- 23,8), GII (442.3 +/- 30.2), and GIV (564.1 +/- 78.7). the levels of progesterone in GIII were superior to those in GI, GII, and GIV.Conclusion(s): Our findings suggest that melatonin may attenuate proliferation in ovarian structures and increase the number of luteal bodies as well as the levels of progesterone receptor. (Fertil Steril (R) 2011;95:1379-84. (C)2011 by American Society for Reproductive Medicine.)
Keywords Melatonin
Ovary
VEGF
PCNA
Rats
Language English
Sponsor Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
Grant number FAPESP: 2006/60412-7
FAPESP: 2007/54398-4
Date 2011-03-01
Published in Fertility and Sterility. New York: Elsevier B.V., v. 95, n. 4, p. 1379-1384, 2011.
ISSN 0015-0282 (Sherpa/Romeo, impact factor)
Publisher Elsevier B.V.
Extent 1379-1384
Origin https://dx.doi.org/10.1016/j.fertnstert.2010.04.042
Access rights Open access Open Access
Type Article
Web of Science ID WOS:000288010900031
URI https://repositorio.unifesp.br/handle/11600/33480

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