Effects of melatonin on histomorphology and on the expression of steroid receptors, VEGF, and PCNA in ovaries of pinealectomized female rats

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dc.contributor.authorRomeu, Lucrecia Regina Gomes [UNIFESP]
dc.contributor.authorMotta, Eduardo Leme Alves da [UNIFESP]
dc.contributor.authorMaganhin, Carla Cristina [UNIFESP]
dc.contributor.authorOshima, Celina Tizuko Fujiyama [UNIFESP]
dc.contributor.authorFonseca, Marcelle Cavalcante [UNIFESP]
dc.contributor.authorBarrueco, Karina Fonseca [UNIFESP]
dc.contributor.authorSimões, Ricardo Santos [UNIFESP]
dc.contributor.authorPellegrino, Renata [UNIFESP]
dc.contributor.authorBaracat, Edmund Chada [UNIFESP]
dc.contributor.authorSoares Júnior, José Maria [UNIFESP]
dc.contributor.institutionUniversidade Federal de São Paulo (UNIFESP)
dc.date.accessioned2016-01-24T14:06:12Z
dc.date.available2016-01-24T14:06:12Z
dc.date.issued2011-03-01
dc.description.abstractObjective: To evaluate the effect of melatonin both on the ovaries of pinealectomized female rats through histomorphometric analysis and on steroid receptors, proliferating cell nuclear antigen (PCNA), and vascular endothelial growth factor (VEGF) expression.Design: Experimental study.Setting: Federal University of São Paulo, Brazil.Animal(s): Forty female rats.Intervention(s): Forty rats were divided equally into four groups: GI-vehicle without surgery; GII-surgery without removal of the pineal gland (sham); GIII-pinealectomized with vehicle; and GIV-pinealectomized with melatonin treatment. After treatment for 3 consecutive months, the animals were killed and their ovaries removed for analysis.Main Outcome Measure(s): Estrogen and progesterone receptors, histologic and immunohistochemical analysis.Result(s): the GIII samples presented signals of proliferation on ovarian surface epithelium and interstitial cells as well as high expressions of PCNA and VEGF in those structures compared with GI, GII, and GIV. Also, the levels of progesterone receptor (fmol/g) in ovaries of GIII (250.6 +/- 32.4) were significantly lower than in those of GI (429.0 +/- 23,8), GII (442.3 +/- 30.2), and GIV (564.1 +/- 78.7). the levels of progesterone in GIII were superior to those in GI, GII, and GIV.Conclusion(s): Our findings suggest that melatonin may attenuate proliferation in ovarian structures and increase the number of luteal bodies as well as the levels of progesterone receptor. (Fertil Steril (R) 2011;95:1379-84. (C)2011 by American Society for Reproductive Medicine.)en
dc.description.affiliationUniversidade Federal de São Paulo, Dept Gynecol, São Paulo, Brazil
dc.description.affiliationUniversidade Federal de São Paulo, Pathol Sect, São Paulo, Brazil
dc.description.affiliationUniversidade Federal de São Paulo, Dept Gynecol & Obstet, São Paulo, Brazil
dc.description.affiliationUniversidade Federal de São Paulo, Dept Psychobiol, São Paulo, Brazil
dc.description.affiliationUnifespUniversidade Federal de São Paulo, Dept Gynecol, São Paulo, Brazil
dc.description.affiliationUnifespUniversidade Federal de São Paulo, Pathol Sect, São Paulo, Brazil
dc.description.affiliationUnifespUniversidade Federal de São Paulo, Dept Gynecol & Obstet, São Paulo, Brazil
dc.description.affiliationUnifespUniversidade Federal de São Paulo, Dept Psychobiol, São Paulo, Brazil
dc.description.sourceWeb of Science
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.description.sponsorshipIDFAPESP: 2006/60412-7
dc.description.sponsorshipIDFAPESP: 2007/54398-4
dc.format.extent1379-1384
dc.identifierhttps://dx.doi.org/10.1016/j.fertnstert.2010.04.042
dc.identifier.citationFertility and Sterility. New York: Elsevier B.V., v. 95, n. 4, p. 1379-1384, 2011.
dc.identifier.doi10.1016/j.fertnstert.2010.04.042
dc.identifier.issn0015-0282
dc.identifier.urihttps://repositorio.unifesp.br/handle/11600/33480
dc.identifier.wosWOS:000288010900031
dc.language.isoeng
dc.publisherElsevier B.V.
dc.relation.ispartofFertility and Sterility
dc.rightsinfo:eu-repo/semantics/openAccess
dc.rights.licensehttp://www.elsevier.com/about/open-access/open-access-policies/article-posting-policy
dc.subjectMelatoninen
dc.subjectOvaryen
dc.subjectVEGFen
dc.subjectPCNAen
dc.subjectRatsen
dc.titleEffects of melatonin on histomorphology and on the expression of steroid receptors, VEGF, and PCNA in ovaries of pinealectomized female ratsen
dc.typeinfo:eu-repo/semantics/article
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