Supplementing alpha-tocopherol (vitamin E) and vitamin D3 in high fat diet decrease IL-6 production in murine epididymal adipose tissue and 3T3-L1 adipocytes following LPS stimulation

Supplementing alpha-tocopherol (vitamin E) and vitamin D3 in high fat diet decrease IL-6 production in murine epididymal adipose tissue and 3T3-L1 adipocytes following LPS stimulation

Author Lira, Fabio Santos de Autor UNIFESP Google Scholar
Rosa, Jose Cesar Autor UNIFESP Google Scholar
Cunha, Claudio Alexandre Autor UNIFESP Google Scholar
Ribeiro, Eliane Beraldi Autor UNIFESP Google Scholar
Nascimento, Claudia Maria da Penha Oller do Autor UNIFESP Google Scholar
Oyama, Lila Missae Autor UNIFESP Google Scholar
Mota, João Felipe Autor UNIFESP Google Scholar
Institution Universidade Federal de São Paulo (UNIFESP)
Abstract Background: It is well known that high fat diets (HFDs) induce obesity and an increase in proinflammatory adipokines. Interleukin-6 (IL-6) is considered the major inflammatory mediator in obesity. Obesity is associated with a vitamin deficiency, especially of vitamins E and D3. We examined the effects of vitamin D3 and vitamin E supplementation on levels of IL-6 and IL-10 (as a marker of anti-inflammatory cytokines since, a balance between pro-and anti-inflammatory cytokines is maintained) protein expression in adipose tissue of mice provided with an HFD. Additionally, we measured the effects of vitamin E and vitamin D3 treatment on LPS-stimulated 3T3-L1 adipocytes IL-6 and IL-10 secretion.Results: IL-6 protein levels and the IL-6/IL-10 ratio were decreased in epididymal white adipose tissue in groups receiving vitamins E and D3 supplementation compared to the HFD group. A 24-hour treatment of vitamin D3 and vitamin E significantly reduced the IL-6 levels in the adipocytes culture medium without affecting IL-10 levels.Conclusions: Vitamin D3 and vitamin E supplementation in an HFD had an anti-inflammatory effect by decreasing IL-6 production in epididymal adipose tissue in mice and in 3T3-L1 adipocytes stimulated with LPS. Our results suggest that vitamin E and D3 supplementation can be used as an adjunctive therapy to reduce the proinflammatory cytokines present in obese patients.
Language English
Sponsor Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
Grant number FAPESP: 07/53444 2
FAPESP: 08/54733 0
Date 2011-02-27
Published in Lipids in Health and Disease. London: Biomed Central Ltd, v. 10, 5 p., 2011.
ISSN 1476-511X (Sherpa/Romeo, impact factor)
Publisher Biomed Central Ltd
Extent 5
Origin https://dx.doi.org/10.1186/1476-511X-10-37
Access rights Open access Open Access
Type Article
Web of Science ID WOS:000288093100001
URI https://repositorio.unifesp.br/handle/11600/33476

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