Supplementing alpha-tocopherol (vitamin E) and vitamin D3 in high fat diet decrease IL-6 production in murine epididymal adipose tissue and 3T3-L1 adipocytes following LPS stimulation

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dc.contributor.authorLira, Fabio Santos de [UNIFESP]
dc.contributor.authorRosa, Jose Cesar [UNIFESP]
dc.contributor.authorCunha, Claudio Alexandre [UNIFESP]
dc.contributor.authorRibeiro, Eliane Beraldi [UNIFESP]
dc.contributor.authorNascimento, Claudia Maria da Penha Oller do [UNIFESP]
dc.contributor.authorOyama, Lila Missae [UNIFESP]
dc.contributor.authorMota, João Felipe [UNIFESP]
dc.contributor.institutionUniversidade Federal de São Paulo (UNIFESP)
dc.date.accessioned2016-01-24T14:06:12Z
dc.date.available2016-01-24T14:06:12Z
dc.date.issued2011-02-27
dc.description.abstractBackground: It is well known that high fat diets (HFDs) induce obesity and an increase in proinflammatory adipokines. Interleukin-6 (IL-6) is considered the major inflammatory mediator in obesity. Obesity is associated with a vitamin deficiency, especially of vitamins E and D3. We examined the effects of vitamin D3 and vitamin E supplementation on levels of IL-6 and IL-10 (as a marker of anti-inflammatory cytokines since, a balance between pro-and anti-inflammatory cytokines is maintained) protein expression in adipose tissue of mice provided with an HFD. Additionally, we measured the effects of vitamin E and vitamin D3 treatment on LPS-stimulated 3T3-L1 adipocytes IL-6 and IL-10 secretion.Results: IL-6 protein levels and the IL-6/IL-10 ratio were decreased in epididymal white adipose tissue in groups receiving vitamins E and D3 supplementation compared to the HFD group. A 24-hour treatment of vitamin D3 and vitamin E significantly reduced the IL-6 levels in the adipocytes culture medium without affecting IL-10 levels.Conclusions: Vitamin D3 and vitamin E supplementation in an HFD had an anti-inflammatory effect by decreasing IL-6 production in epididymal adipose tissue in mice and in 3T3-L1 adipocytes stimulated with LPS. Our results suggest that vitamin E and D3 supplementation can be used as an adjunctive therapy to reduce the proinflammatory cytokines present in obese patients.en
dc.description.affiliationUniversidade Federal de São Paulo, Dept Fis, São Paulo, Brazil
dc.description.affiliationUnifespUniversidade Federal de São Paulo, Dept Fis, São Paulo, Brazil
dc.description.sourceWeb of Science
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.description.sponsorshipIDFAPESP: 07/53444 2
dc.description.sponsorshipIDFAPESP: 08/54733 0
dc.format.extent5
dc.identifierhttps://dx.doi.org/10.1186/1476-511X-10-37
dc.identifier.citationLipids in Health and Disease. London: Biomed Central Ltd, v. 10, 5 p., 2011.
dc.identifier.doi10.1186/1476-511X-10-37
dc.identifier.fileWOS000288093100001.pdf
dc.identifier.issn1476-511X
dc.identifier.urihttps://repositorio.unifesp.br/handle/11600/33476
dc.identifier.wosWOS:000288093100001
dc.language.isoeng
dc.publisherBiomed Central Ltd
dc.relation.ispartofLipids in Health and Disease
dc.rightsinfo:eu-repo/semantics/openAccess
dc.titleSupplementing alpha-tocopherol (vitamin E) and vitamin D3 in high fat diet decrease IL-6 production in murine epididymal adipose tissue and 3T3-L1 adipocytes following LPS stimulationen
dc.typeinfo:eu-repo/semantics/article
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