Low-Level Laser Therapy Associated to N-Acetylcysteine Lowers Macrophage Inflammatory Protein-2 (MIP-2) mRNA Expression and Generation of Intracellular Reactive Oxygen Species in Alveolar Macrophages

Low-Level Laser Therapy Associated to N-Acetylcysteine Lowers Macrophage Inflammatory Protein-2 (MIP-2) mRNA Expression and Generation of Intracellular Reactive Oxygen Species in Alveolar Macrophages

Author Mafra de Lima, F. Google Scholar
Balbin Villaverde, A. Google Scholar
Albertini, R. Google Scholar
Ligeiro de Oliveira, A. P. Google Scholar
Castro Faria Neto, H. C. Google Scholar
Aimbire, F. Autor UNIFESP Google Scholar
Institution UNIVAP
Univ Camilo Castelo Branco UNICASTELO
Ctr Univ Nove de Julho UNINOVE
Inst Biomed Sci
IOC
Universidade Federal de São Paulo (UNIFESP)
Abstract Objective: the aim of this work was to investigate the low-level laser therapy (LLLT) effect on alveolar macrophages (AM) activated by oxidative stress and lipopolysaccharide (LPS). Background data: LLLT has been reported to actuate positively relieving the late and early symptoms of airway and lung inflammation. It is not known if the increased MIP-2mRNA expression and intracellular reactive oxygen species (ROS) generation observed in acute lung inflammation (ALI) can be influenced by LLLT. Materials and Methods: Rat AM cell line (AMJ2-C11) was cultured with LPS or H(2)O(2) and laser irradiated. MIP-2mRNA and ROS production in the AM were evaluated by Real Time-PCR and the 2',7'-dichlorofluorescin diacetate (DCFH-DA) respectively. the NF-kappa B protein in the AM was measured by the enzyme linked immunoassay method. To investigate the antioxidant effect of laser, the AM were prebathed with N-acetylcysteine (NAC) and then irradiated with laser. LLLT was also studied in the presence of an inhibitor of NF-kappa B (BMS 205820). in addition, the effect of LLLT on NF-kappa B protein was investigated. Results: LLLT attenuated the MIP-2mRNA expression and intracellular ROS generation after LPS or H(2)O(2). When the AM were pretreated with NAC, the laser effect was potentiated. BMS 205820 suppresses the effect of LLLT on MIP-2mRNA expression and ROS generation, stimulated by LPS or H(2)O(2). On NF-kappa B transcription factor, both the LLLT and NAC reduced this protein in the AM exposed to LPS or H(2)O(2). the synergistic effect between LLLT and NAC on the reduction the NF-kappa B was also evidenced. Conclusion: Results indicate that there is a synergistic action of LLLT with NAC on MIP-2mRNA expression from LPS-or H(2)O(2)-stimulated AM, and that both ROS intracellular generation and NF-kB signaling seem to be involved.
Language English
Date 2010-12-01
Published in Photomedicine and Laser Surgery. New Rochelle: Mary Ann Liebert Inc, v. 28, n. 6, p. 763-771, 2010.
ISSN 1549-5418 (Sherpa/Romeo, impact factor)
Publisher Mary Ann Liebert Inc
Extent 763-771
Origin http://dx.doi.org/10.1089/pho.2009.2638
Access rights Closed access
Type Article
Web of Science ID WOS:000285189700009
URI http://repositorio.unifesp.br/handle/11600/33147

Show full item record




File

File Size Format View

There are no files associated with this item.

This item appears in the following Collection(s)

Search


Browse

Statistics

My Account