Low-Level Laser Therapy Associated to N-Acetylcysteine Lowers Macrophage Inflammatory Protein-2 (MIP-2) mRNA Expression and Generation of Intracellular Reactive Oxygen Species in Alveolar Macrophages

Página do item simplificado
dc.contributor.authorMafra de Lima, Flavia
dc.contributor.authorVillaverde, Antonio Balbin
dc.contributor.authorAlbertini, Regiane
dc.contributor.authorLigeiro de Oliveira, Ana Paula
dc.contributor.authorCastro-Faria-Neto, Hugo Caire
dc.contributor.authorAimbire, Flavio [UNIFESP]
dc.contributor.institutionUNIVAP
dc.contributor.institutionUniv Camilo Castelo Branco UNICASTELO
dc.contributor.institutionCtr Univ Nove de Julho UNINOVE
dc.contributor.institutionInst Biomed Sci
dc.contributor.institutionIOC
dc.contributor.institutionUniversidade Federal de São Paulo (UNIFESP)
dc.date.accessioned2016-01-24T14:05:47Z
dc.date.available2016-01-24T14:05:47Z
dc.date.issued2010-12-01
dc.description.abstractObjective: the aim of this work was to investigate the low-level laser therapy (LLLT) effect on alveolar macrophages (AM) activated by oxidative stress and lipopolysaccharide (LPS). Background data: LLLT has been reported to actuate positively relieving the late and early symptoms of airway and lung inflammation. It is not known if the increased MIP-2mRNA expression and intracellular reactive oxygen species (ROS) generation observed in acute lung inflammation (ALI) can be influenced by LLLT. Materials and Methods: Rat AM cell line (AMJ2-C11) was cultured with LPS or H(2)O(2) and laser irradiated. MIP-2mRNA and ROS production in the AM were evaluated by Real Time-PCR and the 2',7'-dichlorofluorescin diacetate (DCFH-DA) respectively. the NF-kappa B protein in the AM was measured by the enzyme linked immunoassay method. To investigate the antioxidant effect of laser, the AM were prebathed with N-acetylcysteine (NAC) and then irradiated with laser. LLLT was also studied in the presence of an inhibitor of NF-kappa B (BMS 205820). in addition, the effect of LLLT on NF-kappa B protein was investigated. Results: LLLT attenuated the MIP-2mRNA expression and intracellular ROS generation after LPS or H(2)O(2). When the AM were pretreated with NAC, the laser effect was potentiated. BMS 205820 suppresses the effect of LLLT on MIP-2mRNA expression and ROS generation, stimulated by LPS or H(2)O(2). On NF-kappa B transcription factor, both the LLLT and NAC reduced this protein in the AM exposed to LPS or H(2)O(2). the synergistic effect between LLLT and NAC on the reduction the NF-kappa B was also evidenced. Conclusion: Results indicate that there is a synergistic action of LLLT with NAC on MIP-2mRNA expression from LPS-or H(2)O(2)-stimulated AM, and that both ROS intracellular generation and NF-kB signaling seem to be involved.en
dc.description.affiliationUNIVAP, IP&D, Inst Res & Dev, São Paulo, Brazil
dc.description.affiliationUniv Camilo Castelo Branco UNICASTELO, Inst Biomed Engn, São Paulo, Brazil
dc.description.affiliationCtr Univ Nove de Julho UNINOVE, Rehabil Sci Dept, São Paulo, Brazil
dc.description.affiliationInst Biomed Sci, Dept Pharmacol, São Paulo, Brazil
dc.description.affiliationIOC, Lab Immunopharmacol, Rio de Janeiro, Brazil
dc.description.affiliationSão Paulo Fed Univ UNIFESP, Dept Sci & Technol, São Paulo, Brazil
dc.description.affiliationUnifespSão Paulo Fed Univ UNIFESP, Dept Sci & Technol, São Paulo, Brazil
dc.description.sourceWeb of Science
dc.format.extent763-771
dc.identifierhttps://dx.doi.org/10.1089/pho.2009.2638
dc.identifier.citationPhotomedicine and Laser Surgery. New Rochelle: Mary Ann Liebert Inc, v. 28, n. 6, p. 763-771, 2010.
dc.identifier.doi10.1089/pho.2009.2638
dc.identifier.issn1549-5418
dc.identifier.urihttps://repositorio.unifesp.br/handle/11600/33147
dc.identifier.wosWOS:000285189700009
dc.language.isoeng
dc.publisherMary Ann Liebert Inc
dc.relation.ispartofPhotomedicine and Laser Surgery
dc.rightsinfo:eu-repo/semantics/restrictedAccess
dc.titleLow-Level Laser Therapy Associated to N-Acetylcysteine Lowers Macrophage Inflammatory Protein-2 (MIP-2) mRNA Expression and Generation of Intracellular Reactive Oxygen Species in Alveolar Macrophagesen
dc.typeinfo:eu-repo/semantics/article
Arquivos
Coleções
ICT - Artigos