Acute exercise reverses TRB3 expression in the skeletal muscle and ameliorates whole body insulin sensitivity in diabetic mice

Acute exercise reverses TRB3 expression in the skeletal muscle and ameliorates whole body insulin sensitivity in diabetic mice

Author Matos, A. Google Scholar
Ropelle, E. R. Google Scholar
Pauli, J. R. Autor UNIFESP Google Scholar
Frederico, M. J. S. Google Scholar
Pinho, R. A. de Google Scholar
Velloso, L. A. Google Scholar
De Souza, C. T. Google Scholar
Institution Univ Cruzeiro Sul
Universidade Estadual de Campinas (UNICAMP)
Universidade Federal de São Paulo (UNIFESP)
Univ So Santa Catarina
Abstract Aim:TRB3 became of major interest in diabetes research when it was shown to interact with and inhibit the activity of Akt. Conversely, physical exercise has been linked to improved glucose homeostasis. Thus, the current study was designed to investigate the effects of acute exercise on TRB3 expression and whole body insulin sensitivity in obese diabetic mice.Methods:Male leptin-deficient (ob/ob) mice swam for two 3-h-long bouts, separated by a 45-min rest period. After the second bout of exercise, food was withdrawn 6 h before antibody analysis. Eight hours after the exercise protocol, the mice were submitted to an insulin tolerance test (ITT). Gastrocnemius muscle samples were evaluated for insulin receptor (IR) and IRS-1 tyrosine phosphorylation, Akt serine phosphorylation, TRB3/Akt association and membrane GLUT4 expression.Results:Western blot analysis showed that TRB3 expression was reduced in the gastrocnemius of leptin-deficient (ob/ob) mice submitted to exercise when compared with respective ob/ob mice at rest. in parallel, there was an increase in the insulin-signalling pathway in skeletal muscle from leptin-deficient mice after exercise. Furthermore, the GLUT4 membrane expression was increased in the muscle after the exercise protocol. Finally, a single session of exercise improved the glucose disappearance (K(ITT)) rate in ob/ob mice.Conclusion:Our results demonstrate that acute exercise reverses TRB3 expression and insulin signalling restoration in muscle. Thus, these results provide new insights into the mechanism by which physical activity ameliorates whole body insulin sensitivity in type 2 diabetes.
Keywords exercise
GLUT4 translocation
insulin sensitivity
TRB3 expression
Language English
Sponsor Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
Date 2010-01-01
Published in Acta Physiologica. Malden: Wiley-Blackwell Publishing, Inc, v. 198, n. 1, p. 61-69, 2010.
ISSN 1748-1708 (Sherpa/Romeo, impact factor)
Publisher Wiley-Blackwell
Extent 61-69
Access rights Closed access
Type Article
Web of Science ID WOS:000272566000005

Show full item record


File Size Format View

There are no files associated with this item.

This item appears in the following Collection(s)




My Account