Acute exercise reverses TRB3 expression in the skeletal muscle and ameliorates whole body insulin sensitivity in diabetic mice

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dc.contributor.authorMatos, Agberto Corrêa de
dc.contributor.authorRopelle, Eduardo Rochete
dc.contributor.authorPauli, José Rodrigo [UNIFESP]
dc.contributor.authorFrederico, Marisa Jadna Silva
dc.contributor.authorPinho, Ricardo Aurino de
dc.contributor.authorVelloso, Lício Augusto
dc.contributor.authorSouza, Claudio Teodoro de
dc.contributor.institutionUniv Cruzeiro Sul
dc.contributor.institutionUniversidade Estadual de Campinas (UNICAMP)
dc.contributor.institutionUniversidade Federal de São Paulo (UNIFESP)
dc.contributor.institutionUniv So Santa Catarina
dc.date.accessioned2016-01-24T13:59:09Z
dc.date.available2016-01-24T13:59:09Z
dc.date.issued2010-01-01
dc.description.abstractAim:TRB3 became of major interest in diabetes research when it was shown to interact with and inhibit the activity of Akt. Conversely, physical exercise has been linked to improved glucose homeostasis. Thus, the current study was designed to investigate the effects of acute exercise on TRB3 expression and whole body insulin sensitivity in obese diabetic mice.Methods:Male leptin-deficient (ob/ob) mice swam for two 3-h-long bouts, separated by a 45-min rest period. After the second bout of exercise, food was withdrawn 6 h before antibody analysis. Eight hours after the exercise protocol, the mice were submitted to an insulin tolerance test (ITT). Gastrocnemius muscle samples were evaluated for insulin receptor (IR) and IRS-1 tyrosine phosphorylation, Akt serine phosphorylation, TRB3/Akt association and membrane GLUT4 expression.Results:Western blot analysis showed that TRB3 expression was reduced in the gastrocnemius of leptin-deficient (ob/ob) mice submitted to exercise when compared with respective ob/ob mice at rest. in parallel, there was an increase in the insulin-signalling pathway in skeletal muscle from leptin-deficient mice after exercise. Furthermore, the GLUT4 membrane expression was increased in the muscle after the exercise protocol. Finally, a single session of exercise improved the glucose disappearance (K(ITT)) rate in ob/ob mice.Conclusion:Our results demonstrate that acute exercise reverses TRB3 expression and insulin signalling restoration in muscle. Thus, these results provide new insights into the mechanism by which physical activity ameliorates whole body insulin sensitivity in type 2 diabetes.en
dc.description.affiliationUniv Cruzeiro Sul, São Paulo, Brazil
dc.description.affiliationUniv Estadual Campinas, FCM, Dept Clin Med, Campinas, SP, Brazil
dc.description.affiliationUNIFESP, Dept Biociencias, Curso Educ Fis Modalidade Saude, Santos, SP, Brazil
dc.description.affiliationUniv So Santa Catarina, Postgrad Program Hlth Sci, Exercise Biochem & Physiol Lab, Criciuma, SC, Brazil
dc.description.affiliationUnifespUNIFESP, Dept Biociencias, Curso Educ Fis Modalidade Saude, Santos, SP, Brazil
dc.description.sourceWeb of Science
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)pt
dc.format.extent61-69
dc.identifierhttps://dx.doi.org/10.1111/j.1748-1716.2009.02031.x
dc.identifier.citationActa Physiologica. Malden: Wiley-Blackwell Publishing, Inc, v. 198, n. 1, p. 61-69, 2010.
dc.identifier.doi10.1111/j.1748-1716.2009.02031.x
dc.identifier.issn1748-1708
dc.identifier.urihttps://repositorio.unifesp.br/handle/11600/32140
dc.identifier.wosWOS:000272566000005
dc.language.isoeng
dc.publisherWiley-Blackwell
dc.relation.ispartofActa Physiologica
dc.rightsinfo:eu-repo/semantics/restrictedAccess
dc.rights.licensehttp://olabout.wiley.com/WileyCDA/Section/id-406071.html
dc.subjectExerciseen
dc.subjectGLUT4 translocationen
dc.subjectInsulinen
dc.subjectInsulin sensitivityen
dc.subjectMuscleen
dc.subjectTRB3 expressionen
dc.titleAcute exercise reverses TRB3 expression in the skeletal muscle and ameliorates whole body insulin sensitivity in diabetic miceen
dc.typeinfo:eu-repo/semantics/article
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