The kinin B-2 receptor gene structure, product processing and expression in adult and fetal rats: evidence for gene evolution
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2010-01-01
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Franca, Camilla Estevao de 
Vicari, Camilo Alfredo Faigle
Piza, Ailton Marques
Geroldo, Elaine Aparecida
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We examined the structure of the rat kinin B-2 receptor gene (KB(2)r) and encoding messenger RNA (mRNA) processing. Differently from the closely related mouse and rabbit genes that have three exons and two introns, the rat gene purportedly consists of four exons and three introns. There are two purported gene products; one of them contains an upstream similar to 180-bp open reading frame region (exon-X) potentially expressed as a result of alternative processing. To examine the processing of rat KB(2)r mRNA, cDNA amplicons were generated using primer pairs directed towards 5' or 3' exon or intron flanking regions. Analyses of intron/exon primary cDNA amplicons showed that introns 1 to 3 are removed sequentially and that exon-X removal follows that of intron-3. No evidence was found for exon-X expression in polyadenylated (mature) mRNA of adult Wistar, Wistar Kyoto, spontaneously hypertensive or Sprague-Dawley rat tissues. Nor was exon-X detected in tissues subject to inflammatory stimulus expressing B-1 kinin receptor mRNA or in 1- to 21-day-old rat embryos or fetuses. the lack of evidence for the expression of exon-X in mature mRNA indicates that the structure of the rat gene is similar to that of the mouse, rabbit and human genes, all consisting of three exons and two introns. the exon-X fragment may result from interstitial gene duplication, be a fragment of the ancestral gene, or most likely heterologous transposon insertion of an exon-like fragment into intron-2 of the KB(2)r gene.
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Genetics and Molecular Research. Ribeirao Preto: Funpec-editora, v. 9, n. 1, p. 215-230, 2010.