The kinin B-2 receptor gene structure, product processing and expression in adult and fetal rats: evidence for gene evolution

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dc.contributor.authorFranca, Camilla Estevao de [UNIFESP]
dc.contributor.authorVicari, Camilo Alfredo Faigle [UNIFESP]
dc.contributor.authorPiza, Ailton Marques [UNIFESP]
dc.contributor.authorGeroldo, Elaine Aparecida [UNIFESP]
dc.contributor.authorBeçak, Maria Luiza
dc.contributor.authorBeçak, Willy
dc.contributor.authorStocco, Rita de Cassia
dc.contributor.authorLindsey, Charles Julian [UNIFESP]
dc.contributor.institutionUniversidade Federal de São Paulo (UNIFESP)
dc.contributor.institutionInst Butantan
dc.date.accessioned2016-01-24T13:59:02Z
dc.date.available2016-01-24T13:59:02Z
dc.date.issued2010-01-01
dc.description.abstractWe examined the structure of the rat kinin B-2 receptor gene (KB(2)r) and encoding messenger RNA (mRNA) processing. Differently from the closely related mouse and rabbit genes that have three exons and two introns, the rat gene purportedly consists of four exons and three introns. There are two purported gene products; one of them contains an upstream similar to 180-bp open reading frame region (exon-X) potentially expressed as a result of alternative processing. To examine the processing of rat KB(2)r mRNA, cDNA amplicons were generated using primer pairs directed towards 5' or 3' exon or intron flanking regions. Analyses of intron/exon primary cDNA amplicons showed that introns 1 to 3 are removed sequentially and that exon-X removal follows that of intron-3. No evidence was found for exon-X expression in polyadenylated (mature) mRNA of adult Wistar, Wistar Kyoto, spontaneously hypertensive or Sprague-Dawley rat tissues. Nor was exon-X detected in tissues subject to inflammatory stimulus expressing B-1 kinin receptor mRNA or in 1- to 21-day-old rat embryos or fetuses. the lack of evidence for the expression of exon-X in mature mRNA indicates that the structure of the rat gene is similar to that of the mouse, rabbit and human genes, all consisting of three exons and two introns. the exon-X fragment may result from interstitial gene duplication, be a fragment of the ancestral gene, or most likely heterologous transposon insertion of an exon-like fragment into intron-2 of the KB(2)r gene.en
dc.description.affiliationUniversidade Federal de São Paulo, Dept Biofis, São Paulo, Brazil
dc.description.affiliationInst Butantan, Genet Lab, São Paulo, Brazil
dc.description.affiliationUnifespUniversidade Federal de São Paulo, Dept Biofis, São Paulo, Brazil
dc.description.sourceWeb of Science
dc.format.extent215-230
dc.identifierhttps://dx.doi.org/10.4238/vol9-1gmr681
dc.identifier.citationGenetics and Molecular Research. Ribeirao Preto: Funpec-editora, v. 9, n. 1, p. 215-230, 2010.
dc.identifier.doi10.4238/vol9-1gmr681
dc.identifier.issn1676-5680
dc.identifier.urihttps://repositorio.unifesp.br/handle/11600/32056
dc.identifier.wosWOS:000277326200016
dc.language.isoeng
dc.publisherFunpec-editora
dc.relation.ispartofGenetics and Molecular Research
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subjectAlternative processingen
dc.subjectRat embryosen
dc.subjectKinin B-2 receptor geneen
dc.titleThe kinin B-2 receptor gene structure, product processing and expression in adult and fetal rats: evidence for gene evolutionen
dc.typeinfo:eu-repo/semantics/article
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