Ascorbic acid prevents acute myocardial infarction induced by isoproterenol in rats: role of inducible nitric oxide synthase production

Ascorbic acid prevents acute myocardial infarction induced by isoproterenol in rats: role of inducible nitric oxide synthase production

Author Ribeiro, Daniel Araki Autor UNIFESP Google Scholar
Buttros, Juliana Beatriz Autor UNIFESP Google Scholar
Oshima, Celina Tizuko Fujiyama Autor UNIFESP Google Scholar
Bergamaschi, Cassia Toledo Autor UNIFESP Google Scholar
Campos, Ruy Ribeiro Autor UNIFESP Google Scholar
Institution Universidade Federal de São Paulo (UNIFESP)
Abstract The goal of this study was to investigate whether sub-chronic anti-oxidant treatment with ascorbic acid (Vit C) is able to protect the heart against myocardial infarction. the effects of Vit C treatment on the histopatological changes and immunohistochemistry for p53, COX-2 and iNOS were evaluated in rats submitted to acute myocardial infarction induced by isoproterenol (ISO). Male Wistar rats (n = 32) were divided into four groups: group 1, control; group 2, ISO treated; group 3, Vit C treated; group 4, ISO + Vit C treated. An amount of 150 mg/kg of isoproterenol was administered for two consecutive days. the rats were treated with Vit C once a day (150 mg/kg, orally) for seven consecutive days. in the day 5 and 6 the rats from group ISO + Vit C were submitted to acute administration of ISO third minutes after Vit C treatment. the results pointed out that treatment with Vit C showed mild degenerative changes of myocardial tissue in ISO group. Also, the antioxidant was able to decrease the iNOS expression in rats treated with Vit C. Taken together, our results suggest that chronic Vit C administration was able to prevent the myocardial infarction induced by ISO as a result of iNOS downregulation. Certainly, this finding offers new insights into the mechanisms underlying the relation between oxidative stress and cardiac mortality after myocardial infarction.
Keywords Isoproterenol
Ascorbic acid
Heart infarction
p53
COX-2
iNOS
Rats
Language English
Sponsor Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
Grant number FAPESP: 07/01228-4
Date 2009-04-01
Published in Journal of Molecular Histology. Dordrecht: Springer, v. 40, n. 2, p. 99-105, 2009.
ISSN 1567-2379 (Sherpa/Romeo, impact factor)
Publisher Springer
Extent 99-105
Origin https://dx.doi.org/10.1007/s10735-009-9218-1
Access rights Closed access
Type Article
Web of Science ID WOS:000267784900002
URI https://repositorio.unifesp.br/handle/11600/31409

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