Lycopene activity against chemically induced DNA damage in Chinese hamster ovary cells

Date
2007-08-01Author
Scolastici, Clarissa
Lima, Rodrigo Otavio Alves de
Barbisan, Luis Fernando
Ferreira, Ana Lucia dos Anjos
Ribeiro, Daniel Araki [UNIFESP]
Salvadori, Daisy Maria Favero
Type
ArtigoISSN
0887-2333Is part of
Toxicology in VitroDOI
10.1016/j.tiv.2007.01.020Metadata
Show full item recordAbstract
Lycopene is a natural pigment synthesized by plants and microorganisms, and it is mainly found in tomatoes. It is an acyclic isomer of P-carotene and one of the most potent antioxidants. Several studies have demonstrated the ability of lycopene to prevent chemically induced DNA damage; however, the mechanisms involved are still not clear. in the present study, we investigated the antigenotoxic/antimutagenic effects of lycopene in Chinese Hamster Ovary Cells (CHO) treated with hydrogen peroxide, methylmethanesulphonate (MMS), or 4-nitroquinoline-1-oxide (4-NQO). Lycopene (97%), at final concentrations of 10, 25, and 50 M, was tested under three different protocols: before, simultaneously, and after the treatment with the mutagens. Comet and cytokinesis-block micronucleus assays were used to evaluate the level of DNA damage. Data showed that lycopene reduced the frequency of micronucleated cells induced by the three mutagens. However, this chemopreventive activity was dependent on the concentrations and treatment schedules used. Similar results were observed in the comet assay, although some enhancements of primary DNA damage were detected when the carotenoid was administered after the mutagens. in conclusion, our findings confirmed the chemopreventive activity of lycopene, and showed that this effect occurs under different mechanisms. (c) 2007 Elsevier B.V. All rights reserved.
Citation
Toxicology in Vitro. Oxford: Pergamon-Elsevier B.V., v. 21, n. 5, p. 840-845, 2007.Keywords
LycopeneComet assay
Micronucleus assay
CHO cells
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