Lycopene activity against chemically induced DNA damage in Chinese hamster ovary cells

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dc.contributor.authorScolastici, Clarissa
dc.contributor.authorLima, Rodrigo Otavio Alves de
dc.contributor.authorBarbisan, Luis Fernando
dc.contributor.authorFerreira, Ana Lucia dos Anjos
dc.contributor.authorRibeiro, Daniel Araki [UNIFESP]
dc.contributor.authorSalvadori, Daisy Maria Favero
dc.contributor.institutionSão Paulo State Univ
dc.contributor.institutionUniversidade de São Paulo (USP)
dc.contributor.institutionUniversidade Federal de São Paulo (UNIFESP)
dc.date.accessioned2016-01-24T13:48:56Z
dc.date.available2016-01-24T13:48:56Z
dc.date.issued2007-08-01
dc.description.abstractLycopene is a natural pigment synthesized by plants and microorganisms, and it is mainly found in tomatoes. It is an acyclic isomer of P-carotene and one of the most potent antioxidants. Several studies have demonstrated the ability of lycopene to prevent chemically induced DNA damage; however, the mechanisms involved are still not clear. in the present study, we investigated the antigenotoxic/antimutagenic effects of lycopene in Chinese Hamster Ovary Cells (CHO) treated with hydrogen peroxide, methylmethanesulphonate (MMS), or 4-nitroquinoline-1-oxide (4-NQO). Lycopene (97%), at final concentrations of 10, 25, and 50 M, was tested under three different protocols: before, simultaneously, and after the treatment with the mutagens. Comet and cytokinesis-block micronucleus assays were used to evaluate the level of DNA damage. Data showed that lycopene reduced the frequency of micronucleated cells induced by the three mutagens. However, this chemopreventive activity was dependent on the concentrations and treatment schedules used. Similar results were observed in the comet assay, although some enhancements of primary DNA damage were detected when the carotenoid was administered after the mutagens. in conclusion, our findings confirmed the chemopreventive activity of lycopene, and showed that this effect occurs under different mechanisms. (c) 2007 Elsevier B.V. All rights reserved.en
dc.description.affiliationSão Paulo State Univ, Botucatu Med Sch, Dept Pathol, BR-18518000 São Paulo, Brazil
dc.description.affiliationSão Paulo State Univ, Inst Biosci, Botucatu, SP, Brazil
dc.description.affiliationSão Paulo State Univ, Botucatu Med Sch, Dept Clin Med, São Paulo, Brazil
dc.description.affiliationUniversidade Federal de São Paulo, Dept Hlth Sci, São Paulo, Brazil
dc.description.affiliationUnifespUniversidade Federal de São Paulo, Dept Hlth Sci, São Paulo, Brazil
dc.description.sourceWeb of Science
dc.format.extent840-845
dc.identifierhttps://dx.doi.org/10.1016/j.tiv.2007.01.020
dc.identifier.citationToxicology in Vitro. Oxford: Pergamon-Elsevier B.V., v. 21, n. 5, p. 840-845, 2007.
dc.identifier.doi10.1016/j.tiv.2007.01.020
dc.identifier.issn0887-2333
dc.identifier.urihttps://repositorio.unifesp.br/handle/11600/29917
dc.identifier.wosWOS:000247559300011
dc.language.isoeng
dc.publisherElsevier B.V.
dc.relation.ispartofToxicology in Vitro
dc.rightsAcesso restrito
dc.rights.licensehttp://www.elsevier.com/about/open-access/open-access-policies/article-posting-policy
dc.subjectLycopeneen
dc.subjectComet assayen
dc.subjectMicronucleus assayen
dc.subjectCHO cellsen
dc.titleLycopene activity against chemically induced DNA damage in Chinese hamster ovary cellsen
dc.typeArtigo
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