Rapid detection and identification of metallo-beta-lactamase-encoding genes by multiplex real-time PCR assay and melt curve analysis
Data
2007-02-01
Autores
Mendes, Rodrigo Elisandro 
Kiyota, Katia Akemi
Monteiro, Jussimara
Castanheira, Mariana
Andrade, Soraya Sgambatti
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Metallo-p-lactamase enzymes (MOL) are encoded by transferable genes, which appear to spread rapidly among gram-negative bacteria. the objective of this study was to develop a multiplex real-time PCR assay followed by a melt curve step for rapid detection and identification of genes encoding MOL-type enzymes based on the amplicon melting peak. the reference sequences of all genes encoding IMP and VIM types, SPM-1, GIM-1, and SIM-1 were downloaded from GenBank, and primers were designed to obtain amplicons showing different sizes and melting peak temperatures (T.). the real-time PCR assay was able to detect all M beta L-harboring clinical isolates, and the T-m-assigned genotypes were 100% coincident with previous sequencing results. This assay could be suitable for identification of MOL-producing gram-negative bacteria by molecular diagnostic laboratories.
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Journal of Clinical Microbiology. Washington: Amer Soc Microbiology, v. 45, n. 2, p. 544-547, 2007.