Rapid detection and identification of metallo-beta-lactamase-encoding genes by multiplex real-time PCR assay and melt curve analysis

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dc.contributor.authorMendes, Rodrigo Elisandro [UNIFESP]
dc.contributor.authorKiyota, Katia Akemi [UNIFESP]
dc.contributor.authorMonteiro, Jussimara [UNIFESP]
dc.contributor.authorCastanheira, Mariana [UNIFESP]
dc.contributor.authorAndrade, Soraya Sgambatti [UNIFESP]
dc.contributor.authorGales, Ana Cristina [UNIFESP]
dc.contributor.authorPignatari, Antonio Carlos Campos [UNIFESP]
dc.contributor.authorTufik, Sergio [UNIFESP]
dc.contributor.institutionUniversidade Federal de São Paulo (UNIFESP)
dc.contributor.institutionAFIP
dc.date.accessioned2016-01-24T12:41:50Z
dc.date.available2016-01-24T12:41:50Z
dc.date.issued2007-02-01
dc.description.abstractMetallo-p-lactamase enzymes (MOL) are encoded by transferable genes, which appear to spread rapidly among gram-negative bacteria. the objective of this study was to develop a multiplex real-time PCR assay followed by a melt curve step for rapid detection and identification of genes encoding MOL-type enzymes based on the amplicon melting peak. the reference sequences of all genes encoding IMP and VIM types, SPM-1, GIM-1, and SIM-1 were downloaded from GenBank, and primers were designed to obtain amplicons showing different sizes and melting peak temperatures (T.). the real-time PCR assay was able to detect all M beta L-harboring clinical isolates, and the T-m-assigned genotypes were 100% coincident with previous sequencing results. This assay could be suitable for identification of MOL-producing gram-negative bacteria by molecular diagnostic laboratories.en
dc.description.affiliationUniversidade Federal de São Paulo, Div Infect Dis, Lab Especial Microbiol Clin, BR-04025010 São Paulo, Brazil
dc.description.affiliationUniversidade Federal de São Paulo, Div Infect Dis, Lab ALERTA, BR-04025010 São Paulo, Brazil
dc.description.affiliationAFIP, Med Lab, São Paulo, Brazil
dc.description.affiliationUniversidade Federal de São Paulo, Dept Psychobiol, São Paulo, Brazil
dc.description.affiliationUnifespUniversidade Federal de São Paulo, Div Infect Dis, Lab Especial Microbiol Clin, BR-04025010 São Paulo, Brazil
dc.description.affiliationUnifespUniversidade Federal de São Paulo, Div Infect Dis, Lab ALERTA, BR-04025010 São Paulo, Brazil
dc.description.affiliationUnifespUniversidade Federal de São Paulo, Dept Psychobiol, São Paulo, Brazil
dc.description.sourceWeb of Science
dc.format.extent544-547
dc.identifierhttps://dx.doi.org/10.1128/JCM.01728-06
dc.identifier.citationJournal of Clinical Microbiology. Washington: Amer Soc Microbiology, v. 45, n. 2, p. 544-547, 2007.
dc.identifier.doi10.1128/JCM.01728-06
dc.identifier.fileWOS000244270000045.pdf
dc.identifier.issn0095-1137
dc.identifier.urihttps://repositorio.unifesp.br/handle/11600/29478
dc.identifier.wosWOS:000244270000045
dc.language.isoeng
dc.publisherAmer Soc Microbiology
dc.relation.ispartofJournal of Clinical Microbiology
dc.rightsinfo:eu-repo/semantics/openAccess
dc.titleRapid detection and identification of metallo-beta-lactamase-encoding genes by multiplex real-time PCR assay and melt curve analysisen
dc.typeinfo:eu-repo/semantics/article
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