Cell populations and adhesion molecules expression in conjunctiva before and after bone marrow transplantation

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dc.contributor.author Rojas, B.
dc.contributor.author Cuhna, R.
dc.contributor.author Zafirakis, P.
dc.contributor.author Ramirez, J. M.
dc.contributor.author Lizan-garciia, M.
dc.contributor.author Zhao, T.
dc.contributor.author Foster, C. S.
dc.date.accessioned 2016-01-24T12:38:02Z
dc.date.available 2016-01-24T12:38:02Z
dc.date.issued 2005-09-01
dc.identifier http://dx.doi.org/10.1016/j.exer.2005.02.004
dc.identifier.citation Experimental Eye Research. London: Academic Press Ltd Elsevier B.V., v. 81, n. 3, p. 313-325, 2005.
dc.identifier.issn 0014-4835
dc.identifier.uri http://repositorio.unifesp.br/handle/11600/28445
dc.description.abstract We were interested to analyse the composition of the cellular infiltrate and adhesion molecules expression in the conjunctiva before and at least one hundred days after autologous and allogenic bone marrow transplantation (BMT) and its relation with the presence of dry eye. We used immunohistochemistry on cryopreserved human conjunctiva with monoclonal antibodies to T-lymphocytes (CD3, CD4 and CD8), B-lymphocytes (CD19), macrophages (CD14), natural killer cells (NK, CD57), intercellular adhesion molecule 1 (ICAM-1), E-selectin, vascular cell adhesion molecule-1 (VCAM-1), lymphocyte function associated antigen-1 (LFA-1), very late antigen-4 (VLA-4), interleukin 2 receptor (IL2r, CD25) and HLA-DR. Our autologous recipients had no graft-versus-host disease (GVHD) but allogenic patients had chronic GVHD. After autologous BMT the conjunctiva had significantly more: (1) T lymphocytes (CD3+, CD4+, CD8+) in the epithelium; (2) CD4+ and CD 14+ cells in the stroma; and (3) VLA-4 expression in the stroma than before BMT. After allogenic BMT, the conjunctiva exhibited a significant increase of: (1) CD3+ and CD 14+ cells in the epithelium; (2) T lymphocytes (CD3+, CD4+, CD8+) and CD 14+ cells in the stroma; and (3) VLA-4 and LFA-1 expression in the stroma than before BMT. After the engraftment, the comparison between autologous and allogenic recipients revealed that: (1) there were no significant differences in adhesion molecule expression; (2) the epithelium of autologous recipients had significantly more CD3+ cells; and (3) the stroma of allogenic patients had significantly more CD3+ and CD8+ cells. Among allogenic recipients, CD14+ cells were significantly increased both in the epithelium and in the stroma of patients with signs or symptoms of dry eye in comparison with patients without ocular involvement. Additionally, those having keratoconjunetivitis sicca (KCS) had CD4/CD8 ratios significantly higher than those without KCS.In conclusion, in the conjunctiva after autologous BMT a subclinical cell mediated immune reaction seems to take place. the conjunctivitis of chronic GVHD is complex, with T cells and macrophages dramatically contributing to the process. (c) 2005 Elsevier B.V. All rights reserved. en
dc.format.extent 313-325
dc.language.iso eng
dc.publisher Elsevier B.V.
dc.relation.ispartof Experimental Eye Research
dc.rights Acesso restrito
dc.subject bone marrow transplantation en
dc.subject graft-versus-host disease en
dc.subject dry eye en
dc.subject keratoconjunctivitis sicca en
dc.subject conjunctiva en
dc.subject lymphocytes en
dc.subject adhesion molecules en
dc.title Cell populations and adhesion molecules expression in conjunctiva before and after bone marrow transplantation en
dc.type Artigo
dc.rights.license http://www.elsevier.com/about/open-access/open-access-policies/article-posting-policy
dc.contributor.institution Univ Complutense Madrid
dc.contributor.institution Harvard Univ
dc.contributor.institution Universidade Federal de São Paulo (UNIFESP)
dc.contributor.institution Hosp Gen Albacete
dc.description.affiliation Univ Complutense Madrid, Inst Invest Oftalmol Ramon Castroviejo, Fac Med, E-28040 Madrid, Spain
dc.description.affiliation Harvard Univ, Sch Med, Massachusetts Eye & Ear Infirm, Hilles Immunol Lab,Dept Ophthalmol, Boston, MA 02114 USA
dc.description.affiliation Universidade Federal de São Paulo, Paulista Sch Med, Dept Ophthalmol, São Paulo, Brazil
dc.description.affiliation Hosp Gen Albacete, Dept Prevent Med, Madrid, Spain
dc.description.affiliationUnifesp Universidade Federal de São Paulo, Paulista Sch Med, Dept Ophthalmol, São Paulo, Brazil
dc.identifier.doi 10.1016/j.exer.2005.02.004
dc.description.source Web of Science
dc.identifier.wos WOS:000231967400008



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