Cell populations and adhesion molecules expression in conjunctiva before and after bone marrow transplantation

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dc.contributor.authorRojas, B.
dc.contributor.authorCuhna, R.
dc.contributor.authorZafirakis, P.
dc.contributor.authorRamirez, J. M.
dc.contributor.authorLizan-garciia, M.
dc.contributor.authorZhao, T.
dc.contributor.authorFoster, C. S.
dc.contributor.institutionUniv Complutense Madrid
dc.contributor.institutionHarvard Univ
dc.contributor.institutionUniversidade Federal de São Paulo (UNIFESP)
dc.contributor.institutionHosp Gen Albacete
dc.date.accessioned2016-01-24T12:38:02Z
dc.date.available2016-01-24T12:38:02Z
dc.date.issued2005-09-01
dc.description.abstractWe were interested to analyse the composition of the cellular infiltrate and adhesion molecules expression in the conjunctiva before and at least one hundred days after autologous and allogenic bone marrow transplantation (BMT) and its relation with the presence of dry eye. We used immunohistochemistry on cryopreserved human conjunctiva with monoclonal antibodies to T-lymphocytes (CD3, CD4 and CD8), B-lymphocytes (CD19), macrophages (CD14), natural killer cells (NK, CD57), intercellular adhesion molecule 1 (ICAM-1), E-selectin, vascular cell adhesion molecule-1 (VCAM-1), lymphocyte function associated antigen-1 (LFA-1), very late antigen-4 (VLA-4), interleukin 2 receptor (IL2r, CD25) and HLA-DR. Our autologous recipients had no graft-versus-host disease (GVHD) but allogenic patients had chronic GVHD. After autologous BMT the conjunctiva had significantly more: (1) T lymphocytes (CD3+, CD4+, CD8+) in the epithelium; (2) CD4+ and CD 14+ cells in the stroma; and (3) VLA-4 expression in the stroma than before BMT. After allogenic BMT, the conjunctiva exhibited a significant increase of: (1) CD3+ and CD 14+ cells in the epithelium; (2) T lymphocytes (CD3+, CD4+, CD8+) and CD 14+ cells in the stroma; and (3) VLA-4 and LFA-1 expression in the stroma than before BMT. After the engraftment, the comparison between autologous and allogenic recipients revealed that: (1) there were no significant differences in adhesion molecule expression; (2) the epithelium of autologous recipients had significantly more CD3+ cells; and (3) the stroma of allogenic patients had significantly more CD3+ and CD8+ cells. Among allogenic recipients, CD14+ cells were significantly increased both in the epithelium and in the stroma of patients with signs or symptoms of dry eye in comparison with patients without ocular involvement. Additionally, those having keratoconjunetivitis sicca (KCS) had CD4/CD8 ratios significantly higher than those without KCS.In conclusion, in the conjunctiva after autologous BMT a subclinical cell mediated immune reaction seems to take place. the conjunctivitis of chronic GVHD is complex, with T cells and macrophages dramatically contributing to the process. (c) 2005 Elsevier B.V. All rights reserved.en
dc.description.affiliationUniv Complutense Madrid, Inst Invest Oftalmol Ramon Castroviejo, Fac Med, E-28040 Madrid, Spain
dc.description.affiliationHarvard Univ, Sch Med, Massachusetts Eye & Ear Infirm, Hilles Immunol Lab,Dept Ophthalmol, Boston, MA 02114 USA
dc.description.affiliationUniversidade Federal de São Paulo, Paulista Sch Med, Dept Ophthalmol, São Paulo, Brazil
dc.description.affiliationHosp Gen Albacete, Dept Prevent Med, Madrid, Spain
dc.description.affiliationUnifespUniversidade Federal de São Paulo, Paulista Sch Med, Dept Ophthalmol, São Paulo, Brazil
dc.description.sourceWeb of Science
dc.format.extent313-325
dc.identifierhttp://dx.doi.org/10.1016/j.exer.2005.02.004
dc.identifier.citationExperimental Eye Research. London: Academic Press Ltd Elsevier B.V., v. 81, n. 3, p. 313-325, 2005.
dc.identifier.doi10.1016/j.exer.2005.02.004
dc.identifier.issn0014-4835
dc.identifier.urihttp://repositorio.unifesp.br/handle/11600/28445
dc.identifier.wosWOS:000231967400008
dc.language.isoeng
dc.publisherElsevier B.V.
dc.relation.ispartofExperimental Eye Research
dc.rightsinfo:eu-repo/semantics/restrictedAccess
dc.rights.licensehttp://www.elsevier.com/about/open-access/open-access-policies/article-posting-policy
dc.subjectbone marrow transplantationen
dc.subjectgraft-versus-host diseaseen
dc.subjectdry eyeen
dc.subjectkeratoconjunctivitis siccaen
dc.subjectconjunctivaen
dc.subjectlymphocytesen
dc.subjectadhesion moleculesen
dc.titleCell populations and adhesion molecules expression in conjunctiva before and after bone marrow transplantationen
dc.typeinfo:eu-repo/semantics/article
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