Cell populations and adhesion molecules expression in conjunctiva before and after bone marrow transplantation

Cell populations and adhesion molecules expression in conjunctiva before and after bone marrow transplantation

Autor Rojas, B. Google Scholar
Cuhna, R. Google Scholar
Zafirakis, P. Google Scholar
Ramirez, J. M. Google Scholar
Lizan-garciia, M. Google Scholar
Zhao, T. Google Scholar
Foster, C. S. Google Scholar
Instituição Univ Complutense Madrid
Harvard Univ
Universidade Federal de São Paulo (UNIFESP)
Hosp Gen Albacete
Resumo We were interested to analyse the composition of the cellular infiltrate and adhesion molecules expression in the conjunctiva before and at least one hundred days after autologous and allogenic bone marrow transplantation (BMT) and its relation with the presence of dry eye. We used immunohistochemistry on cryopreserved human conjunctiva with monoclonal antibodies to T-lymphocytes (CD3, CD4 and CD8), B-lymphocytes (CD19), macrophages (CD14), natural killer cells (NK, CD57), intercellular adhesion molecule 1 (ICAM-1), E-selectin, vascular cell adhesion molecule-1 (VCAM-1), lymphocyte function associated antigen-1 (LFA-1), very late antigen-4 (VLA-4), interleukin 2 receptor (IL2r, CD25) and HLA-DR. Our autologous recipients had no graft-versus-host disease (GVHD) but allogenic patients had chronic GVHD. After autologous BMT the conjunctiva had significantly more: (1) T lymphocytes (CD3+, CD4+, CD8+) in the epithelium; (2) CD4+ and CD 14+ cells in the stroma; and (3) VLA-4 expression in the stroma than before BMT. After allogenic BMT, the conjunctiva exhibited a significant increase of: (1) CD3+ and CD 14+ cells in the epithelium; (2) T lymphocytes (CD3+, CD4+, CD8+) and CD 14+ cells in the stroma; and (3) VLA-4 and LFA-1 expression in the stroma than before BMT. After the engraftment, the comparison between autologous and allogenic recipients revealed that: (1) there were no significant differences in adhesion molecule expression; (2) the epithelium of autologous recipients had significantly more CD3+ cells; and (3) the stroma of allogenic patients had significantly more CD3+ and CD8+ cells. Among allogenic recipients, CD14+ cells were significantly increased both in the epithelium and in the stroma of patients with signs or symptoms of dry eye in comparison with patients without ocular involvement. Additionally, those having keratoconjunetivitis sicca (KCS) had CD4/CD8 ratios significantly higher than those without KCS.In conclusion, in the conjunctiva after autologous BMT a subclinical cell mediated immune reaction seems to take place. the conjunctivitis of chronic GVHD is complex, with T cells and macrophages dramatically contributing to the process. (c) 2005 Elsevier B.V. All rights reserved.
Palavra-chave bone marrow transplantation
graft-versus-host disease
dry eye
keratoconjunctivitis sicca
conjunctiva
lymphocytes
adhesion molecules
Idioma Inglês
Data de publicação 2005-09-01
Publicado em Experimental Eye Research. London: Academic Press Ltd Elsevier B.V., v. 81, n. 3, p. 313-325, 2005.
ISSN 0014-4835 (Sherpa/Romeo, fator de impacto)
Publicador Elsevier B.V.
Extensão 313-325
Fonte http://dx.doi.org/10.1016/j.exer.2005.02.004
Direito de acesso Acesso restrito
Tipo Artigo
Web of Science WOS:000231967400008
Endereço permanente http://repositorio.unifesp.br/handle/11600/28445

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