Characterization of the actions of AvTx 7 isolated from Agelaia vicina (Hymenoptera : Vespidae) wasp venom on synaptosomal glutamate uptake and release

Characterization of the actions of AvTx 7 isolated from Agelaia vicina (Hymenoptera : Vespidae) wasp venom on synaptosomal glutamate uptake and release

Author Pizzo, A. B. Google Scholar
Beleboni, R. O. Google Scholar
Fontana, ACK Google Scholar
Ribeiro, A. M. Google Scholar
Miranda, A. Google Scholar
Coutinho-Netto, J. Google Scholar
Santos, W. F. dos Google Scholar
Institution Universidade de São Paulo (USP)
Oregon Hlth Sci Univ
Universidade Federal de São Paulo (UNIFESP)
Abstract It has previously been shown that the denatured crude extract of Agelaia vicina wasp venom inhibits glutamate and GABA uptake in rat cerebral cortex synaptosomes. To identify the components responsible for these effects, the neurotoxin AvTx 7 (molecular weight of 1210 Da) was isolated from A. vicina venom and its effects on glutamate neurotransmission investigated. AvTx 7 inhibits glutamate uptake in a dose-dependent and uncompetitive manner. AvTx 7 was found to stimulate the glutamate release in the presence of calcium and sodium channel blockers, suggesting that its action is not mediated through these channels. AvTx 7 potentiates glutamate release in the presence of K+ channel blockers tetraethylammoniurn and 4-aminopyridine, indicating that the toxin may act through these drugs-sensible K+ channels. We suggest that AvTx 7 can be a valuable tool to enhance our understanding of K+ channels' involvement in the release of glutamate. (C) 2004 Wiley Periodicals, Inc.
Keywords glutamate release
glutamate uptake
potassium channels
neurotoxin
synaptosomes
wasp venom
Language English
Date 2004-01-01
Published in Journal of Biochemical and Molecular Toxicology. Hoboken: John Wiley & Sons Inc, v. 18, n. 2, p. 61-68, 2004.
ISSN 1095-6670 (Sherpa/Romeo, impact factor)
Publisher Wiley-Blackwell
Extent 61-68
Origin http://dx.doi.org/10.1002/jbt.20014
Access rights Closed access
Type Article
Web of Science ID WOS:000221089700001
URI http://repositorio.unifesp.br/handle/11600/27534

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