Characterization of the actions of AvTx 7 isolated from Agelaia vicina (Hymenoptera : Vespidae) wasp venom on synaptosomal glutamate uptake and release

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dc.contributor.authorPizzo, Andrea Baldocchi
dc.contributor.authorBeleboni, Renê de Oliveira
dc.contributor.authorFontana, Andreia Cristina Karklin
dc.contributor.authorRibeiro, Alessandra Mussi
dc.contributor.authorMiranda, Antonio de [UNIFESP]
dc.contributor.authorCoutinho-Netto, Joaquim
dc.contributor.authorSantos, Wagner Ferreira dos
dc.contributor.institutionUniversidade de São Paulo (USP)
dc.contributor.institutionOregon Hlth Sci Univ
dc.contributor.institutionUniversidade Federal de São Paulo (UNIFESP)
dc.date.accessioned2016-01-24T12:34:11Z
dc.date.available2016-01-24T12:34:11Z
dc.date.issued2004-01-01
dc.description.abstractIt has previously been shown that the denatured crude extract of Agelaia vicina wasp venom inhibits glutamate and GABA uptake in rat cerebral cortex synaptosomes. To identify the components responsible for these effects, the neurotoxin AvTx 7 (molecular weight of 1210 Da) was isolated from A. vicina venom and its effects on glutamate neurotransmission investigated. AvTx 7 inhibits glutamate uptake in a dose-dependent and uncompetitive manner. AvTx 7 was found to stimulate the glutamate release in the presence of calcium and sodium channel blockers, suggesting that its action is not mediated through these channels. AvTx 7 potentiates glutamate release in the presence of K+ channel blockers tetraethylammoniurn and 4-aminopyridine, indicating that the toxin may act through these drugs-sensible K+ channels. We suggest that AvTx 7 can be a valuable tool to enhance our understanding of K+ channels' involvement in the release of glutamate. (C) 2004 Wiley Periodicals, Inc.en
dc.description.affiliationUniv São Paulo, Fac Philosophy Sci & Literature, Lab Neurobiol & Venoms, Ribeirao Preto, SP, Brazil
dc.description.affiliationUniv São Paulo, Sch Med, Lab Neurobiol & Venoms, Neurochem Lab, Ribeirao Preto, SP, Brazil
dc.description.affiliationOregon Hlth Sci Univ, Vollum Inst, Portland, OR 97201 USA
dc.description.affiliationUniversidade Federal de São Paulo, Dept Biophys, São Paulo, SP, Brazil
dc.description.affiliationUnifespUniversidade Federal de São Paulo, Dept Biophys, São Paulo, SP, Brazil
dc.description.affiliationUnifespUniversidade Federal de São Paulo, Dept Biophys, São Paulo, SP, Brazil
dc.description.sourceWeb of Science
dc.format.extent61-68
dc.identifierhttps://dx.doi.org/10.1002/jbt.20014
dc.identifier.citationJournal of Biochemical and Molecular Toxicology. Hoboken: John Wiley & Sons Inc, v. 18, n. 2, p. 61-68, 2004.
dc.identifier.doi10.1002/jbt.20014
dc.identifier.issn1095-6670
dc.identifier.urihttps://repositorio.unifesp.br/handle/11600/27534
dc.identifier.wosWOS:000221089700001
dc.language.isoeng
dc.publisherWiley-Blackwell
dc.relation.ispartofJournal of Biochemical and Molecular Toxicology
dc.rightsAcesso restrito
dc.rights.licensehttp://olabout.wiley.com/WileyCDA/Section/id-406071.html
dc.subjectGlutamate releaseen
dc.subjectGlutamate uptakeen
dc.subjectPotassium channelsen
dc.subjectNeurotoxinen
dc.subjectSynaptosomesen
dc.subjectWasp venomen
dc.titleCharacterization of the actions of AvTx 7 isolated from Agelaia vicina (Hymenoptera : Vespidae) wasp venom on synaptosomal glutamate uptake and releaseen
dc.typeArtigo
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