Phenotypic and genotypic characteristics of Escherichia coli strains of non-enteropathogenic E-coli (EPEC) serogroups that carry eae and lack the EPEC adherence factor and shiga toxin DNA probe sequences
Arquivos
Data
2001-03-01
Autores
Vieira, Monica Aparecida Midolli 
Mostrar mais
Tipo
Artigo
Título da Revista
ISSN da Revista
Título de Volume
Resumo
This study was conducted to characterize the virulence potential of 59 Escherichia coli strains carrying eae and lacking the enteropathogenic E. coli adherence factor and Shiga toxin probe sequences. in hybridization studies, all strains carried the locus of enterocyte effacement (LEE)-associated DNA sequences. of the other 15 virulence DNA sequences tested, hly was the most frequent (44.1%); 17 combinations of these sequences were found, but strains carrying eae only (eae profile) were the most frequent (35.6%). Except for 1 cytodetaching strain, all others adhered to HeLa and Caco-2 cells, most of which (similar to 75.0%) showed variations of the localized adherence pattern. Actin accumulation was detected in 75.9% of the nondetaching strains. Most strains had LEE, probably inserted in pheU (49.2%), and presented a nontypeable intimin (83.1%). Translocated intimin receptor-derived DNA sequences correlated with enteropathogenic and enterohemorrhagic E. coli in 61.0% and 32.0% of the strains, respectively. Thirty-five different serotypes were found. Only strains with the eae profile were associated with diarrhea (P = .039).
Descrição
Citação
Journal of Infectious Diseases. Chicago: Univ Chicago Press, v. 183, n. 5, p. 762-772, 2001.