Phenotypic and genotypic characteristics of Escherichia coli strains of non-enteropathogenic E-coli (EPEC) serogroups that carry eae and lack the EPEC adherence factor and shiga toxin DNA probe sequences

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dc.contributor.authorVieira, Monica Aparecida Midolli [UNIFESP]
dc.contributor.authorAndrade, João Ramos da Costa
dc.contributor.authorTrabulsi, Luiz Rachid
dc.contributor.authorRosa, Ana Claudia de Paula
dc.contributor.authorDias, Angela Maria Girardi
dc.contributor.authorRamos, Sonia Regina Testa da Silva
dc.contributor.authorFrankel, Gad
dc.contributor.authorGomes, Tania Aparecida Tardelli [UNIFESP]
dc.contributor.institutionUniversidade Federal de São Paulo (UNIFESP)
dc.contributor.institutionUniversidade de São Paulo (USP)
dc.contributor.institutionUniversidade do Estado do Rio de Janeiro (UERJ)
dc.contributor.institutionUniv London Imperial Coll Sci Technol & Med
dc.date.accessioned2016-01-24T12:31:19Z
dc.date.available2016-01-24T12:31:19Z
dc.date.issued2001-03-01
dc.description.abstractThis study was conducted to characterize the virulence potential of 59 Escherichia coli strains carrying eae and lacking the enteropathogenic E. coli adherence factor and Shiga toxin probe sequences. in hybridization studies, all strains carried the locus of enterocyte effacement (LEE)-associated DNA sequences. of the other 15 virulence DNA sequences tested, hly was the most frequent (44.1%); 17 combinations of these sequences were found, but strains carrying eae only (eae profile) were the most frequent (35.6%). Except for 1 cytodetaching strain, all others adhered to HeLa and Caco-2 cells, most of which (similar to 75.0%) showed variations of the localized adherence pattern. Actin accumulation was detected in 75.9% of the nondetaching strains. Most strains had LEE, probably inserted in pheU (49.2%), and presented a nontypeable intimin (83.1%). Translocated intimin receptor-derived DNA sequences correlated with enteropathogenic and enterohemorrhagic E. coli in 61.0% and 32.0% of the strains, respectively. Thirty-five different serotypes were found. Only strains with the eae profile were associated with diarrhea (P = .039).en
dc.description.affiliationUniversidade Federal de São Paulo, Escola Paulista Med, Dept Microbiol Imunol & Parasitol, BR-04023062 São Paulo, Brazil
dc.description.affiliationUniv São Paulo, Inst Butantan, Lab Especial Microbiol, São Paulo, Brazil
dc.description.affiliationUniv São Paulo, Inst Adolfo Lutz, Secao Bacteriol, São Paulo, Brazil
dc.description.affiliationUniv São Paulo, Hosp Clin, Inst Crianca, São Paulo, Brazil
dc.description.affiliationUniv Estado Rio de Janeiro, Dept Microbiol, Rio de Janeiro, Brazil
dc.description.affiliationUniv London Imperial Coll Sci Technol & Med, Dept Biochem, London, England
dc.description.affiliationUnifespUniversidade Federal de São Paulo, Escola Paulista Med, Dept Microbiol Imunol & Parasitol, BR-04023062 São Paulo, Brazil
dc.description.sourceWeb of Science
dc.format.extent762-772
dc.identifierhttps://dx.doi.org/10.1086/318821
dc.identifier.citationJournal of Infectious Diseases. Chicago: Univ Chicago Press, v. 183, n. 5, p. 762-772, 2001.
dc.identifier.doi10.1086/318821
dc.identifier.fileWOS000166836500011.pdf
dc.identifier.issn0022-1899
dc.identifier.urihttps://repositorio.unifesp.br/handle/11600/26495
dc.identifier.wosWOS:000166836500011
dc.language.isoeng
dc.publisherUniv Chicago Press
dc.relation.ispartofJournal of Infectious Diseases
dc.rightsAcesso aberto
dc.titlePhenotypic and genotypic characteristics of Escherichia coli strains of non-enteropathogenic E-coli (EPEC) serogroups that carry eae and lack the EPEC adherence factor and shiga toxin DNA probe sequencesen
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