Navegando por Palavras-chave "Hypercholesterolemia"
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- ItemAcesso aberto (Open Access)Avaliação das alterações precoces na coróide e esclera ocorridas em coelhos hipercolesterolêmicos: estudo histológico e histomorfométrico(Conselho Brasileiro de Oftalmologia, 2009-02-01) Torres, Rogil José de Almeida [UNIFESP]; Maia, Maurício [UNIFESP]; Noronha, Lucia; Farah, Michel Eid [UNIFESP]; Luchini, Andréa; Brik, Décio; Muccioli, Cristina [UNIFESP]; Précoma, Dalton Bertolin; Universidade Federal de São Paulo (UNIFESP); Universidade Federal dHospital de Olhos Oeste Paulistae São Paulo Serviço de Cirurgia Vitreorretiniana; Pontifícia Universidade Católica do Paraná Departamento de Patologia; Centro Oftalmológico de Curitiba; Hospital Angelina Caron; Pontifícia Universidade Católica do Paraná Departamento de CardiologiaPURPOSE: To demonstrate experimentally, by means of histological and histomorphometric examinations, the sclera and choroid degenerative alterations, which take place at an early stage due to a hypercholesterolemic diet. METHODS: New Zealand rabbits were divided into two groups: CG (control group) of 6 rabbits (6 eyes) received a regular diet for 6 weeks; G1, of 12 rabbits (12 eyes), was first fed a 1% cholesterol diet (Sigma-Aldrich) for 2 weeks and then from the 14th day on a 0.5% cholesterol diet (Sigma-Aldrich). The eyes underwent a histological analysis, stained with hematoxiline-eosine, and a morphometric examination. The histomorphometric analysis was performed in the posterior region, adjacent to the optic disk, and in the peripheral region. RESULTS: The CG presented a mean sclera and choroid thickness of 228.61 ± 31.71 micrometers in the peripheral region, while the thickness in the posterior region was approximately 246.07 ± 25.66 micrometers. In G1, these values were 303.56 ± 44.21 micrometers in the peripheral region and 295.59 ± 62.59 in the posterior region. There was a statistically significant difference in the sclera and choroid thickness between the groups in the peripheral region (p<0.001); however, this difference did not occur in the posterior region (p=0.250). The large number of histiocytes and collagen fibers accounted for the increase of G1 wall thickness in relation to CG. CONCLUSION: This study demonstrated that the hypercholesterolemic diet in rabbits induces a fast increase in the choroid and sclera thickness, mainly due to the increase in the number of histiocytes and collagen fibers.
- ItemAcesso aberto (Open Access)Estudo da protoporfirina IX como marcador fluorescente da aterosclerose(Universidade Federal de São Paulo, 2014-12-17) Silva, Monica Nascimento da [UNIFESP]; Courrol, Lilia Coronato [UNIFESP]; Universidade Federal de São Paulo (UNIFESP)Atherosclerosis is a chronic degenerative disease characterized by the presence of injuries with aspects of plaques or atheromas in arteries of medium and great caliber. It is the primary cause of cardiovascular disease and stroke, that in turn represent the main cause of death for disease in the world. This study searches a potential atherosclerosis biomarker that it can make possible a minimum invasive diagnostics method and of low cost. In this study 29 rabbits of the race New Zeland had been separate in two main groups: the Control Group, where the animals had received normal diet and the Experimental Group, where the animals had received hypercholesterolemic diet (1% of cholesterol). In a first study blood was collected and liver and arteries were extracted. A metabolic profile of rabbits was made after 22, 43 and 64 days of the beginning of the diet. The excised arteries had been analyzed by microscopy for verification of the installation of the atherosclerotic process in the rabbits of 0 the 64 days. In a second experiment the rabbits had been followed up to 89 days after the beginning of the diet. In this study the blood was collected each 20 days and excrements weekly. Our objective was to verify of the variations of the amount of porphyrin extracted with acetone, in the blood and excrements, with the atherosclerosis stage. With excrements a calibration curve for the determination of the best amount of fecal mass for optimum volume of acetone was gotten, for the extraction of the coproporphyrin. Optimum result was gotten with 0,10 g of fecal mass for 400 µL of acetone. For the group 89 days it had the administration of amminolevulinic acid, or ALA, before and after the blood collection. The study with ALA was continued in one third and last experiment with rabbits. The study of the porphyrin extraction showed the increase of porphyrin fluorescence as in the blood as in excrements in the group of the hypercholesterolemic diet induction, following the increase of the values of LDL (lipoprotein of low density) and the increase of the atheroma. Already the use of the ALA, resulted in an increase in the of the protoporphyrin IX (PPIX) emission signal in the blood of the experimental group in relation to the control group. This study identified a potential marker of atherosclerosis.
- ItemAcesso aberto (Open Access)Fatores modificáveis da degeneração macular relacionada à idade(Conselho Brasileiro de Oftalmologia, 2009-06-01) Torres, Rogil José De Almeida [UNIFESP]; Maia, Maurício [UNIFESP]; Muccioli, Cristina [UNIFESP]; Winter, Guilherme; Souza, Greyce Kelly De; Pasqualotto, Luca Rodrigo; Luchini, Andréa; Précoma, Dalton Bertolim; Hospital Angelina Caron; Universidade Federal de São Paulo (UNIFESP); Hospital de Olhos Oeste Paulista Serviço de Cirurgia Vitreorretiniana; Pontifícia Universidade Católica do Paraná; PUCPR; Centro Oftalmológico de Curitiba; PUCPR Departamento de CardiologiaThe authors present the main modifiable risk factors that may trigger and/or worsen age-related macular degeneration. Mechanisms of action related to these factors as well as preventive measures and intervention effectiveness are discussed.