PPG - Neurologia - Neurociências

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https://hdl.handle.net/11600/61157

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Navegando PPG - Neurologia - Neurociências por Orientador(es) "Amaral, Fernanda Gaspar Do [UNIFESP]"

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    Avaliação preliminar da produção de melatonina no tecido adiposo marrom
    (Universidade Federal de São Paulo (UNIFESP), 2019-12-18) Olescuck, Ingrid Fernandes [UNIFESP]; Amaral, Fernanda Gaspar Do [UNIFESP]; http://lattes.cnpq.br/6398402888086154; http://lattes.cnpq.br/1821363446203117; Universidade Federal de São Paulo (UNIFESP)
    Objective: Verify the existence of local melatonin production in brown adipose tissue. Methods: We realized three experimental models. In experiment I the animals were euthanized every three hours during 24 hours, with no challenge to the interscapular brown adipose tissue (BAT IE). In experiment II the animals were exposed to hipercaloric food over 24h or to cold temperature (13°C) during 3 hours before euthanasia at ZT10. In experiment III the animals were euthanized at ZT18 and there was no additional challenge to the tissue. In all three experiments the animals were assigned to three groups: control, pinealectomized (PINX) and pinealectomized with oral melatonin replacement (PINX/MEL). We collected BAT IE from all animals for the analyses. Results: mRNA from TPH1 enzyme was accessed by PCR reaction in control, PINX and PINX/MEL groups. However it was not possible to standardize the PCR and the Western Blot reactions for AANAT and HIOMT enzymes. In contrast, immunohistochemistry showed the presence of the proteins from both these enzymes (AANAT and HIOMT) in samples obtained by experiment III. Also, we were able to verify AANAT activity in BAT IE from samples obtained for tests, but we were not able to perform this procedure in our experimental samples due to technical limitations. Discussion: Despite all the technical challenges, the present data strongly suggests the presence of local melatonin production in BAT IE. However, more studies are needed to fully prove that.