Baupain, A Plant Cysteine Proteinase That Hinders Thrombin-Induced Human Platelet Aggregation
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2012-04-01
Autores
Andrade, Sheila Siqueira 
Silva, Mariana Cristina Cabral
Gouvea, Iuri Estrada
Kondo, Marcia Yuri
Juliano, Maria Aparecida
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Bauninia forficata is trivially known as cow paw, and popularly used in Brazil for treatment of diabetes mellitus. Denominated baupain a cysteine proteinase was purified from B. forficata leaves. In this study, we investigated the baupain effect on aggregation of isolated human platelets in vitro and the results show that baupain hinders thrombin - but not ADP-and collagen-induced platelet aggregation. With synthetic quenched-fluorescent peptides, the kinetics of the cleavage site of human proteinase-activated receptor 1 / 2 / 3 and 4 [PAR-1 / 2 / 3 and 4] by baupain was determined. In conclusion, similar to bromelain and papain, baupain hinders human platelets aggregation, probably through an unspecific cleavage in the Phe-Leu bond of PAR1.
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Protein And Peptide Letters. Sharjah: Bentham Science Publ Ltd, v. 19, n. 4, p. 474-477, 2012.