Author |
Andrade, Sheila Siqueira
![]() ![]() Silva, Mariana Cristina Cabral ![]() ![]() Gouvea, Iuri Estrada ![]() ![]() Kondo, Marcia Yuri ![]() ![]() Juliano, Maria Aparecida ![]() ![]() Sampaio, Misako Uemura ![]() ![]() Oliva, Maria Luzia Vilela ![]() ![]() |
Institution | Universidade Federal de São Paulo (UNIFESP) |
Abstract | Bauninia forficata is trivially known as cow paw, and popularly used in Brazil for treatment of diabetes mellitus. Denominated baupain a cysteine proteinase was purified from B. forficata leaves. In this study, we investigated the baupain effect on aggregation of isolated human platelets in vitro and the results show that baupain hinders thrombin - but not ADP-and collagen-induced platelet aggregation. With synthetic quenched-fluorescent peptides, the kinetics of the cleavage site of human proteinase-activated receptor 1 / 2 / 3 and 4 [PAR-1 / 2 / 3 and 4] by baupain was determined. In conclusion, similar to bromelain and papain, baupain hinders human platelets aggregation, probably through an unspecific cleavage in the Phe-Leu bond of PAR1. |
Keywords |
Bauhinia
cysteine proteinase plant enzyme platelet aggregation thrombin |
Language | English |
Sponsor |
Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) |
Date | 2012-04-01 |
Published in | Protein And Peptide Letters. Sharjah: Bentham Science Publ Ltd, v. 19, n. 4, p. 474-477, 2012. |
ISSN | 0929-8665 (Sherpa/Romeo, impact factor) |
Publisher | Bentham Science Publ Ltd |
Extent | 474-477 |
Origin |
|
Access rights | Closed access |
Type | Article |
Web of Science ID | WOS:000302157400012 |
URI | http://repositorio.unifesp.br/11600/45145 |
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