Baupain, A Plant Cysteine Proteinase That Hinders Thrombin-Induced Human Platelet Aggregation

Baupain, A Plant Cysteine Proteinase That Hinders Thrombin-Induced Human Platelet Aggregation

Author Andrade, Sheila Siqueira Autor UNIFESP Google Scholar
Silva, Mariana Cristina Cabral Autor UNIFESP Google Scholar
Gouvea, Iuri Estrada Autor UNIFESP Google Scholar
Kondo, Marcia Yuri Autor UNIFESP Google Scholar
Juliano, Maria Aparecida Autor UNIFESP Google Scholar
Sampaio, Misako Uemura Autor UNIFESP Google Scholar
Oliva, Maria Luzia Vilela Autor UNIFESP Google Scholar
Institution Universidade Federal de São Paulo (UNIFESP)
Abstract Bauninia forficata is trivially known as cow paw, and popularly used in Brazil for treatment of diabetes mellitus. Denominated baupain a cysteine proteinase was purified from B. forficata leaves. In this study, we investigated the baupain effect on aggregation of isolated human platelets in vitro and the results show that baupain hinders thrombin - but not ADP-and collagen-induced platelet aggregation. With synthetic quenched-fluorescent peptides, the kinetics of the cleavage site of human proteinase-activated receptor 1 / 2 / 3 and 4 [PAR-1 / 2 / 3 and 4] by baupain was determined. In conclusion, similar to bromelain and papain, baupain hinders human platelets aggregation, probably through an unspecific cleavage in the Phe-Leu bond of PAR1.
Keywords Bauhinia
cysteine proteinase
plant enzyme
platelet aggregation
thrombin
Language English
Sponsor Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
Date 2012-04-01
Published in Protein And Peptide Letters. Sharjah: Bentham Science Publ Ltd, v. 19, n. 4, p. 474-477, 2012.
ISSN 0929-8665 (Sherpa/Romeo, impact factor)
Publisher Bentham Science Publ Ltd
Extent 474-477
Origin http://dx.doi.org/10.2174/092986612799789305
Access rights Closed access
Type Article
Web of Science ID WOS:000302157400012
URI http://repositorio.unifesp.br/11600/45145

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