Porous poly (D,L-lactide-co-glycolide) acid/biosilicate((R)) composite scaffolds for bone tissue engineering

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Kido, Hueliton Wilian [UNIFESP]
Brassolatti, Patricia
Tim, Carla Roberta [UNIFESP]
Gabbai-Armelin, Paulo Roberto [UNIFESP]
Magri, Angela Maria Paiva [UNIFESP]
Fernandes, Kelly Rossetti [UNIFESP]
Bossini, Paulo S.
Parizotto, Nivaldo A.
Crovace, Murilo C.
Malavazi, Iran
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This study evaluated the effects of the Biosilicate((R)) and poly (D,L-lactic-co-glycolic) acid composites on bone repair in a tibial bone defect model in rats by means of using histological evaluation (histopathological and morphometric analysis) and gene expression analysis. Eighty male Wistar rats (12weeks old, weighing +/- 300g) were randomly divided into two groups: Biosilicate((R)) group (BG) and Biosilicate((R))/PLGA group (BG/PLGA). Each group was euthanized at 3, 7, 14, and 21days after surgery (n=10 animals per time point). The main findings showed that the incorporation of PLGA into BG had a significant effect on the morphological structure of the material, accelerating mass loss, decreasing the pH and increasing the calcium release. Furthermore, histologic analysis revealed that the BG/PLGA showed increased material degradation, accompanied by higher bone formation compared to BG, after 21days of implantation. In addition, qRT-PCR analysis showed that BG/PLGA induced an upregulation of the osteogenic genes related to BMP4, Runx2, ALP, and OC. These results show that the present BG/PLGA composite may be used as a bone graft for inducing bone repair. (c) 2015 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 105B: 63-71, 2017.
Journal Of Biomedical Materials Research Part B-Applied Biomaterials. Hoboken, v. 105, n. 1, p. 63-71, 2017.