IDENTIFICATION OF SIALIC ACIDS ON THE CELL-SURFACE OF HYPHAE AND YEAST FORMS OF THE HUMAN PATHOGEN PARACOCCIDIOIDES-BRASILIENSIS
| dc.contributor.author | Soares, RMA | |
| dc.contributor.author | Alviano, C. S. | |
| dc.contributor.author | Angluster, J. | |
| dc.contributor.author | Travassos, Luiz Rodolpho [UNIFESP] | |
| dc.contributor.institution | Universidade Federal do Rio de Janeiro (UFRJ) | |
| dc.contributor.institution | Universidade Federal de São Paulo (UNIFESP) | |
| dc.date.accessioned | 2018-06-15T17:22:35Z | |
| dc.date.available | 2018-06-15T17:22:35Z | |
| dc.date.issued | 1993-03-15 | |
| dc.description.abstract | Paracoccidioides brasiliensis, the agent of paracoccidioidomycosis, when grown in a synthetic medium, expresses at the cell surface of both yeast and mycelial forms acidic glycoconjugates containing N-acetylneuraminic acid units. Sialic acids were extracted using mild hydrolytic conditions, and were identified by thin-layer and gas chromatography, standard colorimetry, reaction with periodate-resorcinol and mass spectrometry. Their surface location was inferred from fluorescent-lectin (Limulus polyphemus agglutinin) binding to whole cells abrogated by previous treatment with neuraminidase. Expression of sialic acids on virulent yeast forms of P. brasiliensis (3.7 X 10(6) residues per cell) may inhibit fungal phagocytosis during early infection, when the immunological response is still being built up. | en |
| dc.description.affiliation | UNIV FED RIO DE JANEIRO,INST MICROBIOL,DEPT MICROBIOL GERAL,CCS BLOCO 1,ILHA FUNDAO,BR-21944 RIO JANEIRO,BRAZIL | |
| dc.description.affiliation | ESCOLA PAULISTA MED SCH,DISCIPLINA BIOL CELULAR,BR-04023 SAO PAULO,BRAZIL | |
| dc.description.affiliationUnifesp | ESCOLA PAULISTA MED SCH,DISCIPLINA BIOL CELULAR,BR-04023 SAO PAULO,BRAZIL | |
| dc.description.source | Web of Science | |
| dc.format.extent | 31-34 | |
| dc.identifier | http://www.sciencedirect.com/science/article/pii/037810979390483I | |
| dc.identifier.citation | Fems Microbiology Letters. Amsterdam: Elsevier Science Bv, v. 108, n. 1, p. 31-34, 1993. | |
| dc.identifier.issn | 0378-1097 | |
| dc.identifier.uri | http://repositorio.unifesp.br/handle/11600/43676 | |
| dc.identifier.wos | WOS:A1993KU26800007 | |
| dc.language.iso | eng | |
| dc.publisher | Elsevier B.V. | |
| dc.relation.ispartof | Fems Microbiology Letters | |
| dc.rights | info:eu-repo/semantics/restrictedAccess | |
| dc.rights.license | http://www.elsevier.com/about/open-access/open-access-policies/article-posting-policy | |
| dc.subject | SIALIC ACID | en |
| dc.subject | N-ACETYLNEURAMINIC ACID | en |
| dc.subject | PARACOCCIDIOIDES-BRASILIENSIS | en |
| dc.subject | YEAST FORMS | en |
| dc.title | IDENTIFICATION OF SIALIC ACIDS ON THE CELL-SURFACE OF HYPHAE AND YEAST FORMS OF THE HUMAN PATHOGEN PARACOCCIDIOIDES-BRASILIENSIS | en |
| dc.type | info:eu-repo/semantics/article |