IDENTIFICATION OF SIALIC ACIDS ON THE CELL-SURFACE OF HYPHAE AND YEAST FORMS OF THE HUMAN PATHOGEN PARACOCCIDIOIDES-BRASILIENSIS

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1993-03-15
Autores
Soares, RMA
Alviano, C. S.
Angluster, J.
Travassos, Luiz Rodolpho [UNIFESP]
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Paracoccidioides brasiliensis, the agent of paracoccidioidomycosis, when grown in a synthetic medium, expresses at the cell surface of both yeast and mycelial forms acidic glycoconjugates containing N-acetylneuraminic acid units. Sialic acids were extracted using mild hydrolytic conditions, and were identified by thin-layer and gas chromatography, standard colorimetry, reaction with periodate-resorcinol and mass spectrometry. Their surface location was inferred from fluorescent-lectin (Limulus polyphemus agglutinin) binding to whole cells abrogated by previous treatment with neuraminidase. Expression of sialic acids on virulent yeast forms of P. brasiliensis (3.7 X 10(6) residues per cell) may inhibit fungal phagocytosis during early infection, when the immunological response is still being built up.
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Fems Microbiology Letters. Amsterdam: Elsevier Science Bv, v. 108, n. 1, p. 31-34, 1993.
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