Autonomous growth of BALB/MK keratinocytes transfected with a retroviral vector carrying the human epidermal growth factor gene

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dc.contributor.authorChoudhuri, Jomuna Veronica [UNIFESP]
dc.contributor.authorMathor, Monica B.
dc.contributor.authorSilva, Flavia H. [UNIFESP]
dc.contributor.authorHan, Sang Won [UNIFESP]
dc.contributor.institutionUniversidade Federal de São Paulo (UNIFESP)
dc.contributor.institutionMQA IPEN CNEN
dc.contributor.institutionUniv Fed Rio Grande do Sul
dc.date.accessioned2016-01-24T13:49:20Z
dc.date.available2016-01-24T13:49:20Z
dc.date.issued2008-01-01
dc.description.abstractEpidermal growth factor (EGF), which promotes epidermal regeneration and wound closure, is important for the proliferation and differentiation of epidermal and epithelial tissues in animals. Exogenous EGF is a promising therapeutic agent for wound healing, but its general use is restricted by the limited availability of this protein. in this work, we show that the transfection of mouse BALB/MK keratinocytes, which are totally dependent on EGF for growth and migration, with mature cDNA for human EGF via a retroviral vector abolished the cells requirement for exogenous EGF. the transformed cells had normal morphology and a growth rate that varied according to the source of the retroviral vector used. Keratinocyte transfection with EGF cDNA provides a time- and cost-efficient means of culturing keratinocytes and yields cells that may be useful for skin grafting.en
dc.description.affiliationUniversidade Federal de São Paulo, Ctr Interdisciplinar Terapia Gen, BR-04044010 São Paulo, Brazil
dc.description.affiliationUniversidade Federal de São Paulo, Dept Biofis, BR-04044010 São Paulo, Brazil
dc.description.affiliationMQA IPEN CNEN, São Paulo, Brazil
dc.description.affiliationUniv Fed Rio Grande do Sul, Dept Genet, Porto Alegre, RS, Brazil
dc.description.affiliationUnifespUniversidade Federal de São Paulo, Ctr Interdisciplinar Terapia Gen, BR-04044010 São Paulo, Brazil
dc.description.affiliationUnifespUniversidade Federal de São Paulo, Dept Biofis, BR-04044010 São Paulo, Brazil
dc.description.sourceWeb of Science
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.description.sponsorshipIDFAPESP: 94/5778-7
dc.description.sponsorshipIDFAPESP: 97/03709-6
dc.format.extent824-828
dc.identifierhttp://dx.doi.org/10.1590/S1415-47572008005000009
dc.identifier.citationGenetics and Molecular Biology. Ribeirao Pret: Soc Brasil Genetica, v. 31, n. 4, p. 824-828, 2008.
dc.identifier.doi10.1590/S1415-47572008005000009
dc.identifier.issn1415-4757
dc.identifier.scieloS1415-47572008005000009
dc.identifier.urihttp://repositorio.unifesp.br/handle/11600/30250
dc.identifier.wosWOS:000261856700003
dc.language.isoeng
dc.publisherSoc Brasil Genetica
dc.relation.ispartofGenetics and Molecular Biology
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subjectBALB/MK keratinocytesen
dc.subjectepidermal growth factoren
dc.subjectretroviral vectoren
dc.titleAutonomous growth of BALB/MK keratinocytes transfected with a retroviral vector carrying the human epidermal growth factor geneen
dc.typeinfo:eu-repo/semantics/article
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