Autonomous growth of BALB/MK keratinocytes transfected with a retroviral vector carrying the human epidermal growth factor gene

Choudhuri, Jomuna Veronica [UNIFESP]; Mathor, Monica B.; Silva, Flavia H. [UNIFESP]; Han, Sang Won [UNIFESP]

Autonomous growth of BALB/MK keratinocytes transfected with a retroviral vector carrying the human epidermal growth factor gene

Data

2008-01-01

Autores

Choudhuri, Jomuna Veronica

Mathor, Monica B.

Silva, Flavia H.

Han, Sang Won

Tipo

Artigo

Resumo

Epidermal growth factor (EGF), which promotes epidermal regeneration and wound closure, is important for the proliferation and differentiation of epidermal and epithelial tissues in animals. Exogenous EGF is a promising therapeutic agent for wound healing, but its general use is restricted by the limited availability of this protein. in this work, we show that the transfection of mouse BALB/MK keratinocytes, which are totally dependent on EGF for growth and migration, with mature cDNA for human EGF via a retroviral vector abolished the cells requirement for exogenous EGF. the transformed cells had normal morphology and a growth rate that varied according to the source of the retroviral vector used. Keratinocyte transfection with EGF cDNA provides a time- and cost-efficient means of culturing keratinocytes and yields cells that may be useful for skin grafting.

Citação

Genetics and Molecular Biology. Ribeirao Pret: Soc Brasil Genetica, v. 31, n. 4, p. 824-828, 2008.