Metabolômica e lipidômica no plasma de mulheres soldadoras: exposição ocupacional a elementos potencialmente tóxicos e os impactos na saúde pública
Data
2024-09-27
Autores
Araújo, Alda Neis Miranda de 
Orientadores
Assunção, Nilson Antônio de
Tipo
Tese de doutorado
Título da Revista
ISSN da Revista
Título de Volume
Resumo
Introdução: A exposição ocupacional a elementos potencialmente tóxicos (EPTs) representa um risco significativo à saúde e pode afetar o metabolismo humano. Objetivos: Avaliar o impacto da exposição ocupacional à diferentes EPTs no perfil metabólico e lipídico de mulheres engajadas na atividade de soldagem de joias domiciliar e informal na cidade de Limeira, Brasil. Métodos: Amostras de sangue de 64 participantes foram divididas em grupo Exposto (n = 36) e grupo Controle (n = 28). Os níveis sanguíneos de EPTs foram determinados por ICP-MS, enquanto os metabólitos e lipídios presentes nas amostras de sangue foram analisados por cromatografia liquida acoplado à espectrometria de massa (LC-MS). Os dados obtidos foram analisados empregando o software Progenesis™ QI v2.4, e o Metaboanalyst 6.0. Subsequentemente, foram realizadas análises univariadas, incluindo o teste t de student e a correlação de Spearman, além de análises multivariadas como a Análise de Componentes Principais (PCA). Resultados: Diferenças significativas foram encontradas nas concentrações de Pb (p < 0,001), As (p < 0,055) e Cd (p < 0,003) entre os grupos. Dos features considerados significativos para o estudo, foram identificados 190 metabólitos e 34 lipídeos. A correlação de spearman apontou correlações moderadas (r > 0,3) e significativas (p value < 0,05) para 54 metabólitos e 11 lipídios com os níveis de EPTs, incluindo, L Fenilalanina com Pb, Cd e As; L-Acetilcarnitina com As. Além disso, a análise de enriquecimento de vias revelou enriquecimentos em vias como, o metabolismo de purina, metabolismo de fenilalanina e tirosina, e oxidação de ácidos graxos. Paralelamente, a análise lipidômica destacou vias como distúrbios do transportador ABC, metabolismo de glicerolipídeos e homeostase da glicose, ambas indicando impactos diretos na fisiologia humana. Conclusões: Este estudo revelou metabólitos e lipídios correlacionados com baixas concentrações de Pb, As, Cd, Sb, Sn, Mn e Zn no sangue. A análise de enriquecimento de vias elucidou ainda mais o potencial impacto da exposição a EPTs em processos biológicos, com possíveis implicações para a saúde. Essas descobertas enfatizam a precariedade do trabalho informal, uma realidade de muitos países em desenvolvimento ao redor mundo.
Introduction: Occupational exposure to low levels of potentially toxic elements (PTEs) poses a significant health risk and can affect human metabolism. Objective: To evaluate the impact of occupational exposure to different PTEs on the metabolic and lipid profiles of women engaged in informal and home-based jewelry soldering activities in the city of Limeira, Brazil. Methods: Blood samples from 64 participants were divided into Exposed group (n=36) and Control group (n=28). Blood levels of PTEs were determined by ICP-MS, while metabolites and lipids present in the blood samples were analyzed using liquid chromatography coupled with mass spectrometry (LC-MS). The data obtained were processed using Progenesis™ QI v2.4 software and MetaboAnalyst 6.0. Subsequently, univariate analyses were performed, including Student's t-test and Spearman's correlation, as well as multivariate analyses such as Principal Component Analysis (PCA). Results: Significant differences were found in the concentrations of Pb (p < 0.001), As (p < 0.055), and Cd (p < 0.003) between the groups. Of the features considered significant for the study, 190 metabolites and 34 lipids were identified. Spearman's correlation showed moderate (r ≥ 0.3) and significant (p-value ≤ 0.05) correlations for 54 metabolites and 11 lipids with PTE levels, including L-Phenylalanine with Pb, Cd, and As; and L-Acetylcarnitine with As. Furthermore, pathway enrichment analysis revealed enrichments in pathways such as purine metabolism, phenylalanine and tyrosine metabolism, and fatty acid oxidation. Similarly, lipidomic analysis highlighted pathways such as ABC transporter disorders, glycerolipid metabolism, and glucose homeostasis, all indicating direct impacts on human physiology. Conclusions: This study unveiled specific metabolites and lipids correlated with low levels Pb, As, Cd, Sb, Sn, Mg, and Zn concentrations. The pathway enrichment analysis further elucidated the potential impact of PTEs exposure on biological processes with possible implications for health outcomes. These findings emphasize that the precariousness of informal work, a reality of many developing countries throughout the globe, involves health risks.
Introduction: Occupational exposure to low levels of potentially toxic elements (PTEs) poses a significant health risk and can affect human metabolism. Objective: To evaluate the impact of occupational exposure to different PTEs on the metabolic and lipid profiles of women engaged in informal and home-based jewelry soldering activities in the city of Limeira, Brazil. Methods: Blood samples from 64 participants were divided into Exposed group (n=36) and Control group (n=28). Blood levels of PTEs were determined by ICP-MS, while metabolites and lipids present in the blood samples were analyzed using liquid chromatography coupled with mass spectrometry (LC-MS). The data obtained were processed using Progenesis™ QI v2.4 software and MetaboAnalyst 6.0. Subsequently, univariate analyses were performed, including Student's t-test and Spearman's correlation, as well as multivariate analyses such as Principal Component Analysis (PCA). Results: Significant differences were found in the concentrations of Pb (p < 0.001), As (p < 0.055), and Cd (p < 0.003) between the groups. Of the features considered significant for the study, 190 metabolites and 34 lipids were identified. Spearman's correlation showed moderate (r ≥ 0.3) and significant (p-value ≤ 0.05) correlations for 54 metabolites and 11 lipids with PTE levels, including L-Phenylalanine with Pb, Cd, and As; and L-Acetylcarnitine with As. Furthermore, pathway enrichment analysis revealed enrichments in pathways such as purine metabolism, phenylalanine and tyrosine metabolism, and fatty acid oxidation. Similarly, lipidomic analysis highlighted pathways such as ABC transporter disorders, glycerolipid metabolism, and glucose homeostasis, all indicating direct impacts on human physiology. Conclusions: This study unveiled specific metabolites and lipids correlated with low levels Pb, As, Cd, Sb, Sn, Mg, and Zn concentrations. The pathway enrichment analysis further elucidated the potential impact of PTEs exposure on biological processes with possible implications for health outcomes. These findings emphasize that the precariousness of informal work, a reality of many developing countries throughout the globe, involves health risks.
Descrição
Citação
ARAÚJO, Alda Neis Miranda de. Metabolômica e lipidômica no plasma de mulheres soldadoras: exposição ocupacional a elementos potencialmente tóxicos e os impactos na saúde pública. 2024. 153 f. Tese (Doutorado em Medicina Translacional) – Escola Paulista de Medicina, Universidade Federal de São Paulo, São Paulo, 2024