Intraspecific variation in alkaline phosphatase activity in Phaeodactylum tricornutum (Bacillariophyceae, Bohlin)

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dc.citation.issue3
dc.citation.volume30
dc.contributor.authorLima, Domenica Teixeira de
dc.contributor.authorPiedras, Fernanda Reinhardt
dc.contributor.authorNogueira, Flavia Previero [UNIFESP]
dc.contributor.authorRichard, Eduardo da Cunha
dc.contributor.authorTocci, Bianca Reis Castaldi
dc.contributor.authorBarrera-Alba, José Juan
dc.contributor.authorMoser, Gleyci Aparecida Oliveira
dc.coverageSao Paulo Sp
dc.date.accessioned2020-08-14T13:44:19Z
dc.date.available2020-08-14T13:44:19Z
dc.date.issued2016
dc.description.abstractTo describe potential intraspecific variation in phosphorus incorporation in two strains of Phaeodactylum tricornutum (Bohlin), Ub3 and Ub7, alkaline phosphatase (AP) activity was evaluated via enzyme-labeled fluorescence assay. Analysis using the probe ELF-97 (R) provides individual evaluation, and therefore can determine the nutritional status of inorganic phosphorus in phytoplanktonic cells. Bioassays compared the control treatment to both phosphate-enriched and phosphate-depleted treatments by varying only the phosphate concentration in the media. The P. tricornutum strains exhibited differences in their development when incubated in the phosphate-enriched media. The development of the Ub7 strain differed by exhibiting "luxury uptake" and utilization of organic phosphorus, and the alkaline phosphatase analysis indicated limitations of this clone under such conditions. The Ub7 strain showed higher AP activity, when compared to Ub3, in the P-enriched condition. P. tricornutum presented increases in AP activity and low variation in Surface/Volume ratio, by increasing biovolume and its maximum linear dimension, as strategies for phosphate incorporation. Our results highlight intraspecific differences in alkaline phosphatase activity, and hence differences in the incorporation of organic phosphorus, as the tested species regulated enzymatic activity under different external phosphate concentrations.en
dc.description.affiliationUniv Estado Rio de Janeiro, Fac Oceanog, Lab Ecol & Cult Fitoplancton Marinho, BR-20550900 Rio De Janeiro, RJ, Brazil
dc.description.affiliationUniv Fed Sao Paulo, Dept Ciencias Mar, Campus Baixada Santista, BR-11030400 Santos, SP, Brazil
dc.description.affiliationInst Nacl Pesquisas Espaciais, Km 39, BR-12630000 Cachoeira Paulista, SP, Brazil
dc.description.affiliationUnifespUniv Fed Sao Paulo, Dept Ciencias Mar, Campus Baixada Santista, BR-11030400 Santos, SP, Brazil
dc.description.sourceWeb of Science
dc.format.extent445-454
dc.identifierhttps://dx.doi.org/10.1590/0102-33062016abb0149
dc.identifier.citationActa Botanica Brasilica. Sao Paulo Sp, v. 30, n. 3, p. 445-454, 2016.
dc.identifier.doi10.1590/0102-33062016abb0149
dc.identifier.fileWOS000385543100011.pdf
dc.identifier.issn0102-3306
dc.identifier.urihttps://repositorio.unifesp.br/handle/11600/57587
dc.identifier.wosWOS:000385543100011
dc.language.isoeng
dc.publisherSoc Botanica Brasil
dc.relation.ispartofActa Botanica Brasilica
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subjectAlkaline phosphataseen
dc.subjectAuxotrophyen
dc.subjectMarine diatomen
dc.subjectOrganic phosphorus incorporationen
dc.subjectPhosphate limitationen
dc.titleIntraspecific variation in alkaline phosphatase activity in Phaeodactylum tricornutum (Bacillariophyceae, Bohlin)en
dc.typeinfo:eu-repo/semantics/article
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