Avaliação do potencial angiogênico in vitro de um biovidro derivado de esponja marinha
Arquivos
Data
2023-12-07
Autores
Melo, Victória Pereira de 
Orientadores
Granito, Renata Neves
Tipo
Trabalho de conclusão de curso
Título da Revista
ISSN da Revista
Título de Volume
Resumo
No contexto da engenharia de tecido ósseo, este estudo tem como objetivo principal avaliar o potencial angiogênico de biovidros derivados de esponjas marinhas, comparando-os com a composição do Bioglass 45S5® (biovidro tradicional - BT). O estudo busca contribuir para o avanço da pesquisa em biomateriais, visando aplicações na engenharia de tecido ósseo, com foco na promoção da angiogênese. A pesquisa envolveu a coleta e identificação de esponjas marinhas, a extração da biosílica a partir desses organismos e a produção de biovidros utilizando essa sílica como fonte alternativa. O ensaio de formação de tubo, conduzido com células endoteliais, foi empregado para avaliar o potencial angiogênico dos biovidros. O estudo incluiu uma comparação entre o biovidro derivado de esponja marinha (biovidro marinho - BM) e o BT em termos de formação de tubos e redes vasculares. Os resultados indicam que o biovidro derivado de esponja marinha demonstrou desempenho semelhante aos do BT em vários parâmetros in vitro relacionados à indução da formação de tubos e redes vasculares. No entanto, foi observada uma diferença significativa no período de 5 dias, com relação ao grupo controle. Esses resultados indicam um possível efeito antiangiogênico dos biovidros tradicional e marinho, mas ressalta a necessidade de investigações adicionais para melhor compreensão dos efeitos em diferentes períodos e concentrações, visando otimizar a aplicação desses biovidros na engenharia de tecidos ósseos.
In the context of bone tissue engineering, this study aims to evaluate the angiogenic potential of a bioactive glass derived from marine sponges, comparing it with the composition of Bioglass 45S5® (traditional bioactive glass - BT). The study seeks to contribute to the advancement of research in biomaterials, focused on applications in bone tissue engineering, specifically promoting angiogenesis. The research involved the collection and identification of marine sponges, the extraction of biosilica from these organisms, and the production of bioactive glasses using this silica as an alternative source. The tube formation assay, conducted with endothelial cells, was employed to assess the angiogenic potential of the bioactive glasses. The study included a comparison between the bioactive glass derived from marine sponge (marine bioactive glass - BM) and BT in terms of tube and vascular network formation. The results indicate that the bioactive glass derived from marine sponge demonstrated performance similar to BT in various in vitro parameters related to the induction of tube and vascular network formation. However, a significant difference was observed at the 5-day mark compared to the control group. These results suggest a possible antiangiogenic effect of both traditional and marine bioactive glasses but underscore the need for further investigations to better understand the effects at different time points and concentrations, aiming to optimize the application of these bioactive glasses in bone tissue engineering.
In the context of bone tissue engineering, this study aims to evaluate the angiogenic potential of a bioactive glass derived from marine sponges, comparing it with the composition of Bioglass 45S5® (traditional bioactive glass - BT). The study seeks to contribute to the advancement of research in biomaterials, focused on applications in bone tissue engineering, specifically promoting angiogenesis. The research involved the collection and identification of marine sponges, the extraction of biosilica from these organisms, and the production of bioactive glasses using this silica as an alternative source. The tube formation assay, conducted with endothelial cells, was employed to assess the angiogenic potential of the bioactive glasses. The study included a comparison between the bioactive glass derived from marine sponge (marine bioactive glass - BM) and BT in terms of tube and vascular network formation. The results indicate that the bioactive glass derived from marine sponge demonstrated performance similar to BT in various in vitro parameters related to the induction of tube and vascular network formation. However, a significant difference was observed at the 5-day mark compared to the control group. These results suggest a possible antiangiogenic effect of both traditional and marine bioactive glasses but underscore the need for further investigations to better understand the effects at different time points and concentrations, aiming to optimize the application of these bioactive glasses in bone tissue engineering.
Descrição
Citação
MELO, Victória Pereira de. Avaliação do potencial angiogênico em vitro de um biovidro derivado de esponja marinha. 2023. 31 f. Trabalho de conclusão de curso (Graduação em Bacharelado Interdisciplinar em Ciência e Tecnologia do Mar) - Universidade Federal de São Paulo, Instituto do Mar, Santos, 2023.