Trends in the Evolution of Snake Toxins Underscored by an Integrative Omics Approach to Profile the Venom of the Colubrid Phalotris mertensi

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dc.citation.issue8
dc.citation.volume8
dc.contributor.authorCampos, Pollyanna Fernandes
dc.contributor.authorAndrade-Silva, Debora
dc.contributor.authorZelanis, Andre [UNIFESP]
dc.contributor.authorPaes Leme, Adriana Franco
dc.contributor.authorRocha, Marisa Maria Teixeira
dc.contributor.authorMenezes, Milene Cristina
dc.contributor.authorSerrano, Solange Maria de Toledo
dc.contributor.authorJunqueira-de-Azevedo, Inácio de Loiola Meirelles
dc.coverageOxford
dc.date.accessioned2020-08-14T13:43:56Z
dc.date.available2020-08-14T13:43:56Z
dc.date.issued2016
dc.description.abstractOnly few studies on snake venoms were dedicated to deeply characterize the toxin secretion of animals from the Colubridae family, despite the fact that they represent the majority of snake diversity. As a consequence, some evolutionary trends observed in venom proteins that underpinned the evolutionary histories of snake toxins were based on data from a minor parcel of the clade. Here, we investigated the proteins of the totally unknown venom from Phalotris mertensi (Dipsadinae subfamily), in order to obtain a detailed profile of its toxins and to appreciate evolutionary tendencies occurring in colubrid venoms. By means of integrated omics and functional approaches, including RNAseq, Sanger sequencing, high-resolution proteomics, recombinant protein production, and enzymatic tests, we verified an active toxic secretion containing up to 21 types of proteins. A high content of Kunitz-type proteins and C-type lectins were observed, although several enzymatic components such as metalloproteinases and an L-amino acid oxidase were also present in the venom. Interestingly, an arguable venom component of other species was demonstrated as a true venom protein and named svLIPA (snake venom acid lipase). This finding indicates the importance of checking the actual protein occurrence across species before rejecting genes suggested to code for toxins, which are relevant for the discussion about the early evolution of reptile venoms. Moreover, trends in the evolution of some toxin classes, such as simplification of metalloproteinases and rearrangements of Kunitz and Wap domains, parallel similar phenomena observed in other venomous snake families and provide a broader picture of toxin evolution.en
dc.description.affiliationInst Butantan, Ctr Toxins Immune Response & Cell Signaling CeTIC, Lab Especial Toxinol Aplicada, Sao Paulo, Brazil
dc.description.affiliationUniv Fed Sao Paulo, Dept Ciencia & Tecnol, Sao Jose Dos Campos, Brazil
dc.description.affiliationLab Nacl Biociencias LNBio, Campinas, SP, Brazil
dc.description.affiliationInst Butantan, Lab Herpetol, Sao Paulo, Brazil
dc.description.affiliationUnifespUniv Fed Sao Paulo, Dept Ciencia & Tecnol, Sao Jose Dos Campos, Brazil
dc.description.sourceWeb of Science
dc.description.sponsorshipFundacao de Amparo a Pesquisa do Estado de Sao Paulo
dc.description.sponsorshipCoordenacao de Aperfeicoamento de Pessoal de Nivel Superior
dc.description.sponsorshipIDFAPESP: 13/07467-1
dc.description.sponsorshipIDCAPES: 387422
dc.format.extent2266-2287
dc.identifierhttps://dx.doi.org/10.1093/gbe/evw149
dc.identifier.citationGenome Biology And Evolution. Oxford, v. 8, n. 8, p. 2266-2287, 2016.
dc.identifier.doi10.1093/gbe/evw149
dc.identifier.issn1759-6653
dc.identifier.urihttps://repositorio.unifesp.br/handle/11600/57453
dc.identifier.wosWOS:000382523500001
dc.language.isoeng
dc.publisherOxford Univ Press
dc.relation.ispartofGenome Biology And Evolution
dc.rightsinfo:eu-repo/semantics/restrictedAccess
dc.subjectSnakeen
dc.subjectVenomen
dc.subjectTranscriptomeen
dc.subjectProteomeen
dc.subjectAcid lipaseen
dc.subjectMolecular evolutionen
dc.titleTrends in the Evolution of Snake Toxins Underscored by an Integrative Omics Approach to Profile the Venom of the Colubrid Phalotris mertensien
dc.typeinfo:eu-repo/semantics/article
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