Plasma rico em plaquetas (PRP) por meio de centrífuga de bancada
Arquivos
Data
2011-05-25
Autores
Calixto, Carlos Alberto 
Orientadores
Ferreira, Lydia Masako
Tipo
Dissertação de mestrado
Título da Revista
ISSN da Revista
Título de Volume
Resumo
INTRODUÇÃO: O plasma rico em plaquetas (PRP) é a concentração autóloga de plaquetas em pequeno volume de plasma, obtido por centrifugação do sangue total com anticoagulante, sendo considerada importante fonte de fatores de crescimento (FC). A sua utilização enfatiza a regeneração tecidual. OBJETIVO: Obter o plasma rico em plaquetas (PRP) por meio de centrífuga de bancada. MÉTODOS: Foram coletos 40ml de sangue total de oito voluntários, para 72 testes, divididos em três grupos (grupos controle, de uma centrifugação, e de duas centrifugações), com variação da força de centrifugação em 200, 400 e 800g. RESULTADOS: Verificou-se que a concentração, no Grupo Duas Centrifugações, apresentou média inferior ao mensurado no Grupo Controle. O Grupo Uma Centrifugação, com a força centrifuga relativa de 200g, apresentou os maiores valores de contagem (417.750/μl), bem como as maiores concentrações de plaquetas (99,03%), que equivaleu a 1,99 vezes o aumento no número das plaquetas do GCt, enquanto que forças de centrifugação superiores foram insuficientes para o enriquecimento. CONCLUSÃO: Obteve-se o plasma rico em plaquetas (PRP), a partir de centrifuga de bancada, com maior concentração na força de 200g.
BACKGROUND: Platelet-rich plasma is an autologous concentration of autologous platelets in a small volume of plasma, obtained by centrifugation of the whole blood, and have been considered to be a rich source of growth factors. OBJECTIVE: Establish a method to prepare a platelet-rich plasma by centrifugation. METHODS: In this study 40 ml from the whole blood was obtained from eight volunteers, and seventy-two tests were performed in three groups (control, one spin, and two spins), changing the intensity performing from 200, 400 and 800g, to establish the method that achieves the optimal platelet enrichment and concentration. RESULTS: The optimal platelet concentration enrichment obtained with the group of two spins was inferior than values was obtained with the group of one spin. In those groups of one spin the enrichment of 99.03% of the platelet concentration was obtained with the relative centrifuge force of 200g. CONCLUSION: Platelet-rich plasma with high platelets counts can be prepared using the method of centrifugation, with highest concentration with the force of 200g.
BACKGROUND: Platelet-rich plasma is an autologous concentration of autologous platelets in a small volume of plasma, obtained by centrifugation of the whole blood, and have been considered to be a rich source of growth factors. OBJECTIVE: Establish a method to prepare a platelet-rich plasma by centrifugation. METHODS: In this study 40 ml from the whole blood was obtained from eight volunteers, and seventy-two tests were performed in three groups (control, one spin, and two spins), changing the intensity performing from 200, 400 and 800g, to establish the method that achieves the optimal platelet enrichment and concentration. RESULTS: The optimal platelet concentration enrichment obtained with the group of two spins was inferior than values was obtained with the group of one spin. In those groups of one spin the enrichment of 99.03% of the platelet concentration was obtained with the relative centrifuge force of 200g. CONCLUSION: Platelet-rich plasma with high platelets counts can be prepared using the method of centrifugation, with highest concentration with the force of 200g.
Descrição
Citação
CALIXTO, Carlos Alberto. Plasma rico em plaquetas (PRP) por meio de centrífuga de bancada. 2011. 123 f. Dissertação (Mestrado) - Escola Paulista de Medicina, Universidade Federal de São Paulo (UNIFESP), São Paulo, 2011.